Polo-Like Kinase phospho S482/phospho S486/phospho S490 Antibody | Rockland Immunochemicals 600-401-E12 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Polo-Like Kinase phospho S482/phospho S486/phospho S490 Antibody

catalog :

600-401-E12

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

Xenopus laevis

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-E12

Name :

Anti-Polo-Like Kinase pS482/pS486/pS490 (Rabbit) Antibody - 600-401-E12

Display Name :

Polo-Like Kinase phospho S482/phospho S486/phospho S490 Antibody

Application Note :

Anti-Polo-Like Kinase pS482/pS486/pS490 Antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 120 kDa in size corresponding to PLKK protein phosphorylated at Ser482/486/490 in the appropriate cell lysate or extract.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

PLK1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Polo-like Kinase pS482/pS486/pS490 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392482/486/490.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Anti-Polo-Like Kinase pS482/pS486/pS490 antibody is directed against phophorylated Polo-Like Kinase protein. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity is expected from the following species based on 100% sequence homology: rat, mouse, human, and zebra fish.

Species Reactivity :

Xenopus

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Synonyms :

Serine/threonine-protein kinase PLK1, Plx1

Target Species :

Xenopus

Background :

Polo-Like Kinase pS482/pS486/pS490 detects Polo-Like Kinase proteins. Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK. Anti-Polo-Like Kinase pS482/pS486/pS490 Antibody is ideal for investigators involved in Cell Cycle and Apoptosis research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P53350.1

more info or order :

Rockland Immunochemicals product webpage