PST1 Antibody | Rockland Immunochemicals 600-401-DU0 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PST1 Antibody

catalog :

600-401-DU0

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

Saccharomyces cerevisiae S288C

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-DU0

Name :

Anti-PST1 (RABBIT) Antibody - 600-401-DU0

Display Name :

PST1 Antibody

Application Note :

Anti-Pst1 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 46 kDa in Western Blots of specific cell lysates and tissues.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000

Western Blot Dilution :

1 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

PST1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Pst1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the N-terminus of yeast Pst1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-Pst1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Pst1 from other sources has not been determined.

Species Reactivity :

Yeast

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Pst1 Antibody, HPF2, HPF2, YDR055W, D4214, YD9609.09, Cell wall mannoprotein PST1, Haze protective factor 2

Target Species :

Human

Background :

Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein (Catalog No. 95-151) was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain ( och1 mnn1 mnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q12355

NCBI :

Q12355

Primary Image Name :

Western Blot of Pst1 Antibody

more info or order :

Rockland Immunochemicals product webpage