PKR Antibody | Rockland Immunochemicals 600-401-DP7 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PKR Antibody

catalog :

600-401-DP7

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-DP7

Name :

Anti-PKR (RABBIT) Antibody - 600-401-DP7

Display Name :

PKR Antibody

Application Note :

Anti-PKR Antibody has been tested for use in Western Blotting, Immunohistochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 62 kDa in Western Blots of specific cell lysates and tissues. Positive Controls used A431 Cells, Daudi Cells, THP-1 Cells, and Rat Lung Tissues.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5,000-1:20,000

Immunohistochemistry Dilution :

2.5-5 μg/mL

IF Microscopy Dilution :

20 μg/mL

Western Blot Dilution :

0.5-1 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

EIF2AK2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-PKR antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 14 amino acids near the C-terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-PKR Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with PKR from other sources has not been determined.

Species Reactivity :

Human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

PKR Antibody, PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

Target Species :

Human

Background :

The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P19525

NCBI :

AAP57628

Primary Image Name :

Western Blot of PKR Antibody

more info or order :

Rockland Immunochemicals product webpage