N-RAS Antibody | Rockland Immunochemicals 600-401-DE6 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

N-RAS Antibody

catalog :

600-401-DE6

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-DE6

Name :

Anti-N-RAS (RABBIT) Antibody - 600-401-DE6

Display Name :

N-RAS Antibody

Application Note :

Anti-N-RAS Antibody has been tested for use in ELISA and Western Blotting. Expect a band at approximately 21 kDa in Western Blots using positive control A431 whole cell lysates or other specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:20,000

Western Blot Dilution :

1 and 2 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

NRAS

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-N-RAS antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid peptide near the C-terminus of human N-RAS.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-N-RAS Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. N-RAS antibody is predicted to not cross-react with H-RAS

Species Reactivity :

Human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

N-RAS Antibody, NS6, ALPS4, N-ras, NRAS1, HRAS1, GTPase NRas, Transforming protein N-Ras

Target Species :

Human

Background :

Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P01111

NCBI :

NP_002515

more info or order :

Rockland Immunochemicals product webpage