MEK1 phospho T386 Antibody | Rockland Immunochemicals 600-401-D73 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MEK1 phospho T386 Antibody

catalog :

600-401-D73

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-D73

Name :

Anti-MEK1 pT386 (Rabbit) Antibody - 600-401-D73

Display Name :

MEK1 phospho T386 Antibody

Application Note :

Anti-MEK1 pT386 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to the MEK1 protein phosphorylated at Thr386 in the appropriate cell lysate or extract.

Buffer :

0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Clonality :

Polyclonal

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

MAP2K1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-MEK1 pT386 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr 386 conjugated to KLH.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Anti-MEK1 pT386 antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. The immunolabeling is completely eliminated by treatment with lambda-phosphatase. Expect reactivity with the following species based on sequence homology: bovine, canine, chicken, mouse, non-human primates and Xenopus. Cross reactivity with MEK1 pT386 from other species has not been determined.

Species Reactivity :

human, rat

Stabilizer :

0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Synonyms :

Dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1

Target Species :

Human

Background :

MEK1 pT386 antibody is directed against human MEK 1 phosphorylated against Thr386. MEK also known as MAP Kinase Kinase and MKK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386. MEK1 pT386 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q02750

NCBI :

AAI39730.1

Primary Image Name :

Western Blot of Anti-MEK1 pT386 (Rabbit) Antibody - 600-401-D73

more info or order :

Rockland Immunochemicals product webpage