Collagen I alpha 1 propeptide Antibody | Rockland Immunochemicals 600-401-D19 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Collagen I alpha 1 propeptide Antibody

catalog :

600-401-D19

quantity :

100 µL

price :

366.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Rockland Immunochemicals product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:250	Rickelt S, Hynes R. Antibodies and methods for immunohistochemistry of extracellular matrix proteins. Matrix Biol. 2018;71-72:10-27 pubmed publisher
	Tian C, Huang Y, Clauser K, Rickelt S, Lau A, Carr S, et al. Suppression of pancreatic ductal adenocarcinoma growth and metastasis by fibrillar collagens produced selectively by tumor cells. Nat Commun. 2021;12:2328 pubmed publisher

product information

Catalog Number :

600-401-D19

Name :

Anti-Collagen I, alpha 1 propeptide (Rabbit) Antibody - 600-401-D19

Display Name :

Collagen I alpha 1 propeptide Antibody

Application Note :

Anti-Collagen I, alpha 1 propeptide Antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 180 kDa in size corresponding to collagen I alpha I polypeptide in human lung fibroblast extracts or other appropriate tissues or lysates. Collagen I alpha 1 propeptide antibody also works well for IHC on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

Titrated value sufficient to run approximately 10 mini blots.

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:5000-1:50,000

Immunohistochemistry Dilution :

1:100

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

COL1A1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-collagen I, alpha 1 propeptide Antibody was produced in rabbit by repeated immunizations with a synthetic peptide corresponding to amino acid residues specific to collagen 1, alpha 1 propeptide conjugated to KLH.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Anti-Collagen I alpha 1 propeptide antibody is directed against collagen I alpha 1 propeptide (C-terminal region). Anti-Collagen I alpha 1 propeptide antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Anti-Collagen I alpha 1 propeptide antibody recognizes Collagen I alpha 1 propeptide from human, rat, and mouse sources. Cross reactivity with Collagen I alpha 1 propeptide from other species has not been determined. However, reactivity is also expected from most vertebrate species based on very high sequence homology.

Species Reactivity :

Human, mouse, rat

Storage :

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Synonyms :

Alpha-1 type I collagen, COLA1A1

Target Species :

Human

Background :

Collagen I alpha 1 propeptide Antibody detects collagen I which is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype. Anti-Collagen I alpha 1 propeptide Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

366.00 USD

UniProt :

P02452

Primary Image Name :

Immunofluorescence Microscopy of Rabbit Anti-Collagen I-alpha 1-propeptide Antibody

more info or order :

Rockland Immunochemicals product webpage