N4BP1 Antibody | Rockland Immunochemicals 600-401-CX1 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

N4BP1 Antibody

catalog :

600-401-CX1

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-CX1

Name :

Anti-N4BP1 (RABBIT) Antibody - 600-401-CX1

Display Name :

N4BP1 Antibody

Application Note :

Anti-N4BP1 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 100 kDa in Western Blots of specific cell lysates and tissues.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5,000

IF Microscopy Dilution :

20 μg/mL

Western Blot Dilution :

.5 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

N4BP1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-N4BP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide near the N-terminus of human N4BP1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-N4BP1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.

Species Reactivity :

Human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

N4BP1 Antibody, KIAA0615, NEDD4-binding protein 1, N4BP1

Target Species :

Human

Background :

Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,IF Microscopy,Western Blot,

Other :

Immunocytochemistry: 10 μg/mL

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O75113

NCBI :

NP_694574

more info or order :

Rockland Immunochemicals product webpage