JMJD2B Antibody | Rockland Immunochemicals 600-401-CB7 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

JMJD2B Antibody

catalog :

600-401-CB7

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-CB7

Name :

Anti-JMJD2B (RABBIT) Antibody - 600-401-CB7

Display Name :

JMJD2B Antibody

Application Note :

Anti-JMJD2B Antibody has been tested for use in ELISA, Western Blotting, and Immunofluorescence. Expect a band at approximately 122 kDa in Western Blots using positive control A-20 whole cell lysate, or other specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:20,000

IF Microscopy Dilution :

20 µg/mL

Western Blot Dilution :

1-2 μg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

KDM4B

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-JMJD2B antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the internal region of human JMJD2B.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-JMJD2B Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with JMJD2B from other sources has not been determined.

Species Reactivity :

Human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

JMJD2B Antibody, JMJD2B, TDRD14B, JHDM3B, JMJD2B, KIAA0876, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B

Target Species :

Human

Background :

Members of the Jumonji domain 2 (JMJD2) family contain a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. Like other members of the JMJD1 and JMJD2 families, JMJD2B is a histone demethylase, but is reported to be a very poor enzyme. Similar to JMJD1A, JMJD2B is a transcriptional target of the hypoxia-inducible factor (HIF), and hypoxic cells express elevated levels of the JMJD2B mRNA and protein. Both JMJD1A and JMJD2B retain their histone demethylase activity in hypoxic conditions, suggesting that this activity of JMJD2B might play a role in the hypoxic gene expression program.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O94953

NCBI :

AAI36612

Primary Image Name :

Western Blot of JMJD2B Antibody

more info or order :

Rockland Immunochemicals product webpage