p90 RSK1 Antibody | Rockland Immunochemicals 600-401-C66 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p90 RSK1 Antibody

catalog :

600-401-C66

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-C66

Name :

Anti-p90 RSK1 (RABBIT) Antibody - 600-401-C66

Display Name :

p90 RSK1 Antibody

Application Note :

Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 protein is expected in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.2 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5000-1:50,000

Western Blot Dilution :

1µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

RPS6KA1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-p90 RSK1 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-p90 RSK1 antibody, rabbit anti-RSK1 antibody, Ribosomal S6 Kinase 1, RSK-1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, p90-RSK 1, p90S6K, MAPKAP kinase 1a, MAPKAPK-1a, Ribosomal S6 kinase 1, RSK 1, RPS6KA1, MAPKAPK1A

Target Species :

Human

Background :

Ribosomal S6 Kinase 1 (RSK1) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q15418

NCBI :

NP_001006666.1

Primary Image Name :

Western blot - Anti-p90 RSK1

more info or order :

Rockland Immunochemicals product webpage