HICE1 pS70 Antibody | Rockland Immunochemicals 600-401-C52 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

HICE1 pS70 Antibody

catalog :

600-401-C52

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-C52

Name :

Anti-Hice1 pS70 (Rabbit) Antibody - 600-401-C52

Display Name :

HICE1 pS70 Antibody

Application Note :

Hice1 pS70 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.22 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:17,000-1:45,000

Western Blot Dilution :

1.22 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

HAUS8

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Hice1 pS70 affinity purified antibody is directed against the phosphorylated form of human Hice1 protein at the S70 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-HICE1 pS70 antibody, HICE-1, HICE 1, HAUS8, HAUS-8, HAUS 8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48

Target Species :

Human

Background :

Hice1 pS70 is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gamma TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9BT25

NCBI :

NP_219485.1

Primary Image Name :

Anti-Hice1 pS70 (Rabbit) Antibody - Western Bolt

more info or order :

Rockland Immunochemicals product webpage