product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
ATG13 phospho S318 Antibody
catalog :
600-401-C49
quantity :
100
price :
https://www.rockland.com/search/?searchString=600-401-C49
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1500; fig 4
Puente C, Hendrickson R, Jiang X. Nutrient-regulated Phosphorylation of ATG13 Inhibits Starvation-induced Autophagy. J Biol Chem. 2016;291:6026-35 pubmed publisher
Brobbey C, Yin S, Liu L, Ball L, Howe P, Delaney J, et al. Autophagy dictates sensitivity to PRMT5 inhibitor in breast cancer. Sci Rep. 2023;13:10752 pubmed publisher
Martínez Martínez E, Atzei P, Vionnet C, Roubaty C, Kaeser Pebernard S, Naef R, et al. A Dual-Acting Nitric Oxide Donor and Phosphodiesterase 5 Inhibitor Activates Autophagy in Primary Skin Fibroblasts. Int J Mol Sci. 2022;23: pubmed publisher
Kallergi E, Daskalaki A, Kolaxi A, Camus C, Ioannou E, Mercaldo V, et al. Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice. Nat Commun. 2022;13:680 pubmed publisher
Munson M, Mathai B, Ng M, Trachsel Moncho L, de la Ballina L, Schultz S, et al. GAK and PRKCD are positive regulators of PRKN-independent mitophagy. Nat Commun. 2021;12:6101 pubmed publisher
Mercer T, Ohashi Y, Boeing S, Jefferies H, De Tito S, Flynn H, et al. Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy. EMBO J. 2021;40:e105985 pubmed publisher
Carinci M, Testa B, Bordi M, Milletti G, Bonora M, Antonucci L, et al. TFG binds LC3C to regulate ULK1 localization and autophagosome formation. EMBO J. 2021;40:e103563 pubmed publisher
Humbert M, Seiler K, Mosimann S, Rentsch V, Sharma K, Pandey A, et al. Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy. Cell Death Differ. 2021;28:2465-2481 pubmed publisher
Ryu H, Kim L, Jeong H, Yeo B, Lee J, Nam H, et al. GSK3B induces autophagy by phosphorylating ULK1. Exp Mol Med. 2021;53:369-383 pubmed publisher
Ren H, Bakas N, Vamos M, Chaikuad A, Limpert A, Wimer C, et al. Design, Synthesis, and Characterization of an Orally Active Dual-Specific ULK1/2 Autophagy Inhibitor that Synergizes with the PARP Inhibitor Olaparib for the Treatment of Triple-Negative Breast Cancer. J Med Chem. 2020;63:14609-14625 pubmed publisher
Tsang T, Posimo J, Gudiel A, Cicchini M, Feldser D, Brady D. Copper is an essential regulator of the autophagic kinases ULK1/2 to drive lung adenocarcinoma. Nat Cell Biol. 2020;22:412-424 pubmed publisher
Azimi M, Oemisch M, Womelsdorf T. Dissociation of nicotinic α7 and α4/β2 sub-receptor agonists for enhancing learning and attentional filtering in nonhuman primates. Psychopharmacology (Berl). 2020;237:997-1010 pubmed publisher
Jia R, Bonifacino J. Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3. elife. 2019;8: pubmed publisher
Fuqua J, Mere C, Kronemberger A, Blomme J, Bae D, Turner K, et al. ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle. FASEB J. 2019;33:11735-11745 pubmed publisher
Wang B, Maxwell B, Joo J, Gwon Y, Messing J, Mishra A, et al. ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Mol Cell. 2019;74:742-757.e8 pubmed publisher
Thayer J, Awad O, Hegdekar N, Sarkar C, Tesfay H, Burt C, et al. The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability. Autophagy. 2019;:1-14 pubmed publisher
Luhr M, Torgersen M, Szalai P, Hashim A, Brech A, Staerk J, et al. The kinase PERK and the transcription factor ATF4 play distinct and essential roles in autophagy resulting from tunicamycin-induced ER stress. J Biol Chem. 2019;294:8197-8217 pubmed publisher
Izumi H, Li Y, Shibaki M, Mori D, Yasunami M, Sato S, et al. Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region. Sci Rep. 2019;9:2236 pubmed publisher
Wirth M, Tooze S. Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-Throughput Screening. Methods Mol Biol. 2019;1880:375-387 pubmed publisher
Jeong Y, Simoneschi D, Keegan S, Melville D, Adler N, Saraf A, et al. The ULK1-FBXW5-SEC23B nexus controls autophagy. elife. 2018;7: pubmed publisher
Wang C, Wang H, Zhang D, Luo W, Liu R, Xu D, et al. Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy. Nat Commun. 2018;9:3492 pubmed publisher
Colecchia D, Dapporto F, Tronnolone S, Salvini L, Chiariello M. MAPK15 is part of the ULK complex and controls its activity to regulate early phases of the autophagic process. J Biol Chem. 2018;293:15962-15976 pubmed publisher
Hagey D, Klum S, Kurtsdotter I, Zaouter C, Topcic D, Andersson O, et al. SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs. PLoS Genet. 2018;14:e1007224 pubmed publisher
Baron O, Boudi A, Dias C, Schilling M, Nölle A, Vizcay Barrena G, et al. Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration. Curr Biol. 2017;27:3626-3642.e6 pubmed publisher
Wallot Hieke N, Verma N, Schlütermann D, Berleth N, Deitersen J, Böhler P, et al. Systematic analysis of ATG13 domain requirements for autophagy induction. Autophagy. 2018;14:743-763 pubmed publisher
Stork B, Dengjel J. Study of ULK1 Catalytic Activity and Its Regulation. Methods Enzymol. 2017;587:391-404 pubmed publisher
Xie Y, Kang R, Tang D. Assessment of Posttranslational Modifications of ATG proteins. Methods Enzymol. 2017;587:171-188 pubmed publisher
Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch A, Muller S, et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. elife. 2017;6: pubmed publisher
Park J, Jung C, Seo M, Otto N, Grunwald D, Kim K, et al. The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14. Autophagy. 2016;12:547-64 pubmed publisher
Hieke N, Löffler A, Kaizuka T, Berleth N, Böhler P, Drießen S, et al. Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. Autophagy. 2015;11:1471-83 pubmed publisher
Egan D, Chun M, Vamos M, Zou H, Rong J, Miller C, et al. Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates. Mol Cell. 2015;59:285-97 pubmed publisher
Jiao H, Su G, Dong W, Zhang L, Xie W, Yao L, et al. Chaperone-like protein p32 regulates ULK1 stability and autophagy. Cell Death Differ. 2015;22:1812-23 pubmed publisher
Rosenberg L, Lafitte M, Grant W, Chen W, Cleveland J, Duckett D. Development of an HTS-Compatible Assay for the Discovery of Ulk1 Inhibitors. J Biomol Screen. 2015;20:913-20 pubmed publisher
Nazio F, Strappazzon F, Antonioli M, Bielli P, Cianfanelli V, Bordi M, et al. mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6. Nat Cell Biol. 2013;15:406-16 pubmed publisher
Stanton M, Dutta S, Zhang H, Polavaram N, Leontovich A, Hönscheid P, et al. Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance. Cancer Res. 2013;73:160-71 pubmed publisher
product information
Catalog Number :
600-401-C49
Name :
Anti-ATG13 pS318 (RABBIT) Antibody - 600-401-C49
Display Name :
ATG13 phospho S318 Antibody
Application Note :
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human phosphorylated ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.2
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100
Default Unit :
µg
ELISA Dilution :
1:25,000-1:175,000
Flow Cytometry Dilution :
User Optimized
Western Blot Dilution :
1:2000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Gene Name :
ATG13
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-ATG13 pS318 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This affinity-purified antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot.  A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known.
Species Reactivity :
Human
Tested Applications :
Dot Blot; ELISA; WB
Suggested Applications :
FC; IF; Multiplex
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-ATG13 pS318 Antibody, ATG-13, ATG 13, Autophagy-related protein 13, KIAA0652
Target Species :
Human
Background :
ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.
Immunogen Type :
Conjugated Peptide
Low Endotoxin :
No
Post Translational Modification :
Phosphorylation
Sample Size :
No
Target Domain of Reactivity :
Internal
Application Text :
ELISA,Flow Cytometry,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2024 Price :
$475.00
UniProt :
O75143
NCBI :
NP_001136145.1
Primary Image Name :
Anti-Human ATG13 pS318 Antibody - Western Blot
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.