product summary
Loading...
company name :
Rockland Immunochemicals
product type :
antibody
product name :
BrdU Antibody
catalog :
600-401-C29
quantity :
100
price :
449.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
citations: 21
Reference
Ning K, Zou W, Xu P, Cheng F, Zhang E, Zhang Chen A, et al. Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors. Sci Adv. 2023;9:eade0869 pubmed publisher
Li D, Xie L, Qiao Z, Zhu J, Yao H, Qin Y, et al. IFI16 Isoforms with Cytoplasmic and Nuclear Locations Play Differential Roles in Recognizing Invaded DNA Viruses. J Immunol. 2021;207:2699-2709 pubmed publisher
Kint S, Van Criekinge W, Vandekerckhove L, De Vos W, Bomsztyk K, Krause D, et al. Single cell epigenetic visualization assay. Nucleic Acids Res. 2021;49:e43 pubmed publisher
Iskusnykh I, Fattakhov N, Buddington R, Chizhikov V. Intrauterine growth restriction compromises cerebellar development by affecting radial migration of granule cells via the JamC/Pard3a molecular pathway. Exp Neurol. 2021;336:113537 pubmed publisher
Kim H, Lee D, Kim E, Jeong I, Kim S, Kim B, et al. Notch Signaling Controls Oligodendrocyte Regeneration in the Injured Telencephalon of Adult Zebrafish. Exp Neurobiol. 2020;29:417-424 pubmed publisher
Horiguchi K, Yoshida S, Tsukada T, Nakakura T, Fujiwara K, Hasegawa R, et al. Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cells. J Reprod Dev. 2020;66:515-522 pubmed publisher
Liu B, Ye B, Zhu X, Yang L, Li H, Liu N, et al. An inducible circular RNA circKcnt2 inhibits ILC3 activation to facilitate colitis resolution. Nat Commun. 2020;11:4076 pubmed publisher
Chizhikov V, Iskusnykh I, Steshina E, Fattakhov N, Lindgren A, Shetty A, et al. Early dorsomedial tissue interactions regulate gyrification of distal neocortex. Nat Commun. 2019;10:5192 pubmed publisher
Roy A, Ghosh A, Kumar B, Chandran B. IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP. elife. 2019;8: pubmed publisher
Velichko A, Petrova N, Luzhin A, Strelkova O, Ovsyannikova N, Kireev I, et al. Hypoosmotic stress induces R loop formation in nucleoli and ATR/ATM-dependent silencing of nucleolar transcription. Nucleic Acids Res. 2019;: pubmed publisher
Sheng C, Mendler I, Rieke S, Snyder P, Jentsch M, Friedrich D, et al. PCNA-Mediated Degradation of p21 Coordinates the DNA Damage Response and Cell Cycle Regulation in Individual Cells. Cell Rep. 2019;27:48-58.e7 pubmed publisher
Sarmento M, Oneto M, Pelicci S, Pesce L, Scipioni L, Faretta M, et al. Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures. Nat Commun. 2018;9:3415 pubmed publisher
Parisotto M, Grelet E, El Bizri R, Dai Y, Terzic J, Eckert D, et al. PTEN deletion in luminal cells of mature prostate induces replication stress and senescence in vivo. J Exp Med. 2018;215:1749-1763 pubmed publisher
Ganaie S, Chen A, Huang C, Xu P, Kleiboeker S, Du A, et al. RNA Binding Protein RBM38 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19, Which Facilitates Viral DNA Replication. J Virol. 2018;92: pubmed publisher
Hagey D, Klum S, Kurtsdotter I, Zaouter C, Topcic D, Andersson O, et al. SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs. PLoS Genet. 2018;14:e1007224 pubmed publisher
Narayan R, Gasol A, Slangen P, Cornelissen F, Lagerweij T, Veldman H, et al. Identification of MEK162 as a Radiosensitizer for the Treatment of Glioblastoma. Mol Cancer Ther. 2018;17:347-354 pubmed publisher
Xu M, Horrell J, Snitow M, Cui J, Gochnauer H, Syrett C, et al. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation. Nat Commun. 2017;8:15397 pubmed publisher
Xu P, Zhou Z, Xiong M, Zou W, Deng X, Ganaie S, et al. Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway. PLoS Pathog. 2017;13:e1006266 pubmed publisher
Shin M, Beane T, Quillien A, Male I, Zhu L, Lawson N. Vegfa signals through ERK to promote angiogenesis, but not artery differentiation. Development. 2016;143:3796-3805 pubmed
Shen W, Deng X, Zou W, Engelhardt J, Yan Z, Qiu J. Analysis of cis and trans Requirements for DNA Replication at the Right-End Hairpin of the Human Bocavirus 1 Genome. J Virol. 2016;90:7761-77 pubmed publisher
Velichko A, Petrova N, Razin S, Kantidze O. Mechanism of heat stress-induced cellular senescence elucidates the exclusive vulnerability of early S-phase cells to mild genotoxic stress. Nucleic Acids Res. 2015;43:6309-20 pubmed publisher
product information
Catalog Number :
600-401-C29
Name :
Anti-BrdU (RABBIT) Antibody - 600-401-C29
Display Name :
BrdU Antibody
Application Note :
Anti-BrdU Antibody has been tested by ELISA, WB, IHC, and is suitable for immunofluorescence microscopy and flow cytometry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated BrdU thymidine analog from replicated cells.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.1 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100
Default Unit :
µg
ELISA Dilution :
1:2000 - 1:10,000
Flow Cytometry Dilution :
1:50-1:100
Immunohistochemistry Dilution :
1:100-1:500
IF Microscopy Dilution :
1:500
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Species Reactivity :
BrdU
Tested Applications :
ELISA; IHC; WB
Suggested Applications :
FC; IF
Storage :
Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-BrdU Antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine
Background :
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2023 Price :
449.00 USD
Primary Image Name :
Immunofluorescence Microscopy
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.