ORAI1 Antibody | Rockland Immunochemicals 600-401-C00 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ORAI1 Antibody

catalog :

600-401-C00

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-C00

Name :

Anti-ORAI1 (Rabbit) Antibody - 600-401-C00

Display Name :

ORAI1 Antibody

Application Note :

ORAI1 antibody has been tested in ELISA, WB, and IHC. ORAI1 often migrates at a higher than expected (~32 kDa) molecular weight in SDS-PAGE. Expect a band at ~51 kDa in size corresponding to ORAI1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:40,000

Immunohistochemistry Dilution :

10 µg/mL

Western Blot Dilution :

0.5 - 1.0 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

ORAI1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

ORAI1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide near the n-terminus of human ORAI1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-ORAI1 Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with ORAI1 with Human and Mouse based on 100% homology with the immunizing sequence. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3. Cross-reactivity with ORAI1 from other sources has not been determined.

Species Reactivity :

Human, mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

ORAI1, Transmembrane protein 142A, TMEM142A, Calcium release-activated calcium channel protein 1, CRACM1

Target Species :

Human

Background :

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. Recently identified, ORAI1 is a four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. Anti-ORAI1 antibody is ideal for investigators involved cell cycle protein and enzyme research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q96D31

NCBI :

Q96D31.2

Primary Image Name :

Anti-ORAI1 Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage