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company name :
Rockland Immunochemicals
product type :
antibody
product name :
IL-27 Antibody
catalog :
600-401-BW1
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
600-401-BW1
Name :
Anti-IL-27 (RABBIT) Antibody - 600-401-BW1
Display Name :
IL-27 Antibody
Application Note :
Anti-IL-27 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 28 kDa in Western Blots of specific cell lysates and tissues.
Buffer :
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:20,000-1:40,000
Western Blot Dilution :
2-4 μg/mL
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
IL27
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-IL-27 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the N-terminus of human IL-27.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.02% (w/v) Sodium Azide
Purity and Specificity :
Anti-IL-27 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with IL-27 from other sources has not been determined.
Species Reactivity :
Human, mouse
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
IL-27 Antibody, p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha
Target Species :
Human
Background :
Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
N-Terminus
Application Text :
ELISA,Western Blot,
Other :
Immunocytochemistry: 10 μg/mL
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q8NEV9
NCBI :
NP_663634
Primary Image Name :
Western Blot of IL-27 Antibody
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.