UBE2J2 Antibody | Rockland Immunochemicals 600-401-B43 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

UBE2J2 Antibody

catalog :

600-401-B43

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-B43

Name :

Anti-Ubiquitin-Conjugating Enzyme E2 J2 (Ube2j2) (RABBIT) Antibody - 600-401-B43

Display Name :

UBE2J2 Antibody

Application Note :

This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29 kDa in size corresponding to Ube2j2 protein by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.91 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:5,000 - 1:25,000

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:500 - 1:3,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

UBE2J2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human Ube2j2 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human Ube2j2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Ube2j2 protein from human, horse, opossum, cattle, dog, mouse, rat, macaque, salmon, chicken, zebra finch, Xenopus and platypus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Species Reactivity :

human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-UBE2J2 antibody, rabbit anti-E2 J2 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J2 antibody, Non-canonical ubiquitin-conjugating enzyme 2, Ube2j2, E2 ubiquitin-conjugating enzyme J2, NCUBE-2

Target Species :

Human

Background :

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j2 and Ube2j1 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal trans-membrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q8N2K1

NCBI :

251757431

Primary Image Name :

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs).

more info or order :

Rockland Immunochemicals product webpage