c-Myb Antibody | Rockland Immunochemicals 600-401-B06 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

c-Myb Antibody

catalog :

600-401-B06

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-B06

Name :

Anti-c-Myb (RABBIT) Antibody - 600-401-B06

Display Name :

c-Myb Antibody

Application Note :

This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of c-Myb should be optimized by the end user. Expect a band approximately ~75 kDa in size corresponding to c-Myb by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.51 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:20,000

Western Blot Dilution :

1:2,500

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Myb

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of mouse c-Myb protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.

Species Reactivity :

Mouse, monkey

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-c-Myb Antibody, Proto-oncogene c-Myb antibody, C myb antibody, MYB antibody, Transcriptional activator Myb, cmyb antibody

Target Species :

Mouse

Background :

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P06876

NCBI :

110556654

Primary Image Name :

Anti-C-Myb Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage