PDCD1 Antibody | Rockland Immunochemicals 600-401-A72 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PDCD1 Antibody

catalog :

600-401-A72

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-A72

Name :

Anti-PDCD1 (RABBIT) Antibody - 600-401-A72

Display Name :

PDCD1 Antibody

Application Note :

This Anti-PDCD1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~47 kDa in size corresponding to PDCD1 by western blotting in the appropriate cell lysate or extract. THP-1 cell lysate can be used as positive control.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:40,000

Immunohistochemistry Dilution :

5.0 µg/mL

Western Blot Dilution :

0.5 - 1.0 µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

PDCD1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human PDCD1 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human PDCD1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with PDCD1 from human, mouse and rat sources. Reactivity with PDCD1 from other sources has not been determined.

Species Reactivity :

human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

PD1 antibody, PDCD 1 antibody, CD279 antibody

Target Species :

Human

Background :

Cell-mediated immune responses are initiated by T lymphocytes th Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 that mediate inhibitory signals from the APC. PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PDCD1 inhibit the activation of the immune response in the absence of “danger signals” such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PDCD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PDCD1 often migrates at higher molecular weight in SDS-PAGE.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q15116

NCBI :

NP_005009.2

Primary Image Name :

Anti-PDCD1 Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage