PDL1 Antibody | Rockland Immunochemicals 600-401-A71 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PDL1 Antibody

catalog :

600-401-A71

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-A71

Name :

Anti-Programmed Cell Death 1 Ligand (PDL-1) (RABBIT) Antibody - 600-401-A71

Display Name :

PDL1 Antibody

Application Note :

Rabbit Anti-PDL-1 antibody has been tested for use in ELISA, IHC, IF, and western blotting. Expect a band at ~32 kDa in size corresponding to PDL-1 by western blotting in the appropriate cell lysate or extract. Raji cell lysate can be used as positive control. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:40,000

Flow Cytometry Dilution :

User Optimized

IF Microscopy Dilution :

User Optimized

Western Blot Dilution :

0.5 - 1 µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CD274

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

PDL-1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to an internal region of human PDL-1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.02% (w/v) Sodium Azide

Purity and Specificity :

Anti-PDL-1 Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with PD-L1 with Human, Rat, and Mouse based on 100% homology with the immunizing sequence. PDL-1 antibody has no cross-reactivity to PDL-2. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. Cross-reactivity with PDL-1 from other sources has not been determined.

Species Reactivity :

human, mouse, rat

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Rabbit anti-PDL1 antibody, Rabbit Anti-Programmed cell death 1 ligand 1 Antibody, Programmed cell death ligand 1, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, PD-L1, PDCD1 ligand 1, B7-H1, B7 homolog 1, PDL-1, PDL 1

Target Species :

Human

Background :

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 (Programmed Cell Death 1) that mediate inhibitory signals from the APC. PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2 (Programmed Cell Death Ligand -1 and -2, respectively). PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. Anti-PDL-1 antibody is ideal for investigators involved in cancer research, cytokine research, immune research and apoptosis research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Flow Cytometry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9NZQ7

NCBI :

Q9NZQ7.1

Primary Image Name :

Immunohistochemistry of PDL-1

more info or order :

Rockland Immunochemicals product webpage