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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Sars Nucleocapsid Protein Antibody
catalog :
600-401-A50
quantity :
100
price :
449.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
SARS coronavirus
application :
western blot, ELISA, immunohistochemistry, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
citations: 4
Reference
V kovski P, Gultom M, Kelly J, Steiner S, Russeil J, Mangeat B, et al. Disparate temperature-dependent virus-host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium. PLoS Biol. 2021;19:e3001158 pubmed publisher
Liu Y, Hu G, Wang Y, Ren W, Zhao X, Ji F, et al. Functional and genetic analysis of viral receptor ACE2 orthologs reveals a broad potential host range of SARS-CoV-2. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Grant B, Anderson C, Williford J, Alonzo L, Glukhova V, Boyle D, et al. SARS-CoV-2 Coronavirus Nucleocapsid Antigen-Detecting Half-Strip Lateral Flow Assay Toward the Development of Point of Care Tests Using Commercially Available Reagents. Anal Chem. 2020;92:11305-11309 pubmed publisher
Zhao B, Ni C, Gao R, Wang Y, Yang L, Wei J, et al. Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids. Protein Cell. 2020;: pubmed publisher
image
image 1 :
Rockland Immunochemicals 600-401-A50 image 1
Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection. Vero E6 cells were either infected with the SARS-CoV-2 Washington isolate (INF) at an MOI of 0.1 or uninfected (UNI) for 24 hours. The cells were then fixed in 4% PFA and stained overnight at 4ºC with primary antibodies directed against SARS-CoV Nucleocapsid (N) at 1:1000 dilution. Imaged using an anti-rabbit secondary conjugated to AlexaFluor 568 [red] and Nuclear Counterstain DAPI [blue]. Image Courtesy of Mohsan Saeed Lab/Da-Yuan Chen, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University.
product information
Catalog Number :
600-401-A50
Name :
Anti-SARS-CoV Nucleocapsid (N) Protein (RABBIT) Antibody - 600-401-A50
Display Name :
Sars Nucleocapsid Protein Antibody
Application Note :
Anti-SARS-CoV-1 purified antibody has been tested for use in ELISA, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding to SARS Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. This antibody can be useful in ELISA and lateral flow format to detect virus in extracts from nasal and throat swabs and saliva. IF can be been useful to determine the presence or absence of virus entering cells especially when anti-viral drugs are applied. IHC studies can be performed on biopsies, detecting the coronavirus in lung, liver, bile duct, and placenta tissue. And may have the ability to neutralize the virus and thereby protect cells from the uptake of live virus. This antibody may be useful in flow cytometry studies.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.2 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100
Default Unit :
µg
ELISA Dilution :
1:10,000 - 1:50,000
Flow Cytometry Dilution :
User Optimized
Immunohistochemistry Dilution :
User Optimized
IF Microscopy Dilution :
User Optimized
Western Blot Dilution :
1:500 - 1:2,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus Nucleocapsid protein.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-SARS-CoV-1 (N) affinity purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 (N) resin. Detects the Nucleocapsid (N), Omicron, and BA.2 sub-variant. BLAST analysis was used to suggest reactivity with related Coronavirus proteins.  Cross reactivity with homologues from other sources has not been determined.
Species Reactivity :
SARS Coronavirus Nucleocapsid
Tested Applications :
ELISA; IF
Suggested Applications :
Other
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-Sars Nucleocapsid Protein Antibody, rabbit anti-Sars-CoV Nucleocapsid (N) Protein Antibody, N antibody, N structural protein antibody, COVID19 antibody, Nucleocapsid protein antibody, Nucleoprotein antibody, SARS coronavirus N protein antibody, SARS CoV-2 antibody, SARSCoV2 antibody, Severe acute respiratory syndrome antibody, A50 Antibody, A50 SARS, COVID
Target Species :
SARS
Background :
The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid.  Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three-domain structure for the nucleocapsid protein. Anti-SARS-CoV Nucleocapsid (N) Protein Antibody is useful for researchers interested in viral research.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Western Blot,
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2023 Price :
449.00 USD
UniProt :
P59595
NCBI :
1489678
Primary Image Name :
Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.