Rock-2 phospho Y256 Antibody | Rockland Immunochemicals 600-401-998 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Rock-2 phospho Y256 Antibody

catalog :

600-401-998

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Reference
Herhaus L, Al Salihi M, MacArtney T, Weidlich S, Sapkota G. OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3. Nat Commun. 2013;4:2519 pubmed publisher

product information

Catalog Number :

600-401-998

Name :

Anti-ROCK-2 (Rho-associated Protein Kinase-2) pY256 (RABBIT) Antibody - 600-401-998

Display Name :

Rock-2 phospho Y256 Antibody

Application Note :

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human ROCK-2 pY256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:2,000 - 1:20,000

Immunohistochemistry Dilution :

User Optimized

Western Blot Dilution :

1:200 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

ROCK2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human ROCK-2 protein phosphorylated at Y256. A BLAST analysis was used to suggest cross-reactivity with ROCK-2 from human, mouse, rat, chicken, dog, bovine, Xenopus and Drosophila based on a 100% homology with the immunizing sequence. Cross-reactivity with ROCK-2 from other sources has not been determined.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-ROCK2 antibody, Rho-associated protein kinase 2, Rho-associated coiled-coil-containing protein kinase 2, p164 ROCK-2, Rho kinase 2, Rho-associated coiled-coil-containing protein kinase II, ROCK-II, ROCK-2, ROCK 2

Target Species :

Human

Background :

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases. Increased levels of RhoA or RhoC, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O75116

NCBI :

4520225

Primary Image Name :

Anti-ROCK-2 pY256 Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage