p44 Map Kinase Antibody | Rockland Immunochemicals 600-401-943 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

p44 Map Kinase Antibody

catalog :

600-401-943

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-943

Name :

Anti-p44 MAP Kinase (ERK1) (RABBIT) Antibody - 600-401-943

Display Name :

p44 Map Kinase Antibody

Application Note :

This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 44 kDa in size corresponding to p44 MAP Kinase (ERK1) by western blotting in the appropriate cell lysate or extract.

Buffer :

0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:2,000 to 1:10,000

Immunoprecipitation Dilution :

5.0 µg/mg sample

Western Blot Dilution :

1:1,000-1:,2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

MAPK3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p44 MAP Kinase (ERK1) protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity-purified antibody is directed against the human p44 MAP Kinase (ERK1) protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p44 MAP Kinase (ERK1) proteins from human, bovine, rabbit, rat and mouse. No reactivity is observed against p42 MAP Kinase (ERK2). Reactivity against homologues from other sources is not known.

Species Reactivity :

mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

ERK1 antibody, ERT 2 antibody, ERT2 antibody, Extracellular Signal Regulated Kinase 1 antibody, Extracellular signal related kinase 1 antibody, HGNC6877 antibody, HS44KDAP antibody, HUMKER1A antibody

Target Species :

Human

Background :

Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK’s) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK’s form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P27361

NCBI :

91718899

Primary Image Name :

Anti-p44 MAP Kinase (ERK1) Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage