product summary
Loading...
company name :
Rockland Immunochemicals
product type :
antibody
product name :
Histone H2AvD phosphoS137 Antibody
catalog :
600-401-914
quantity :
100
price :
509.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
fruit fly
application :
western blot, ELISA, immunohistochemistry
more info or order :
citations: 60
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
| |
Ma X, Han Y, Song X, Do T, Yang Z, Ni J, et al. DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation. Development. 2016;143:4312-4323 pubmed
| |
product information
Catalog Number :
600-401-914
Name :
Anti-Histone H2AvD pS137 (RABBIT) Antibody - 600-401-914
Display Name :
Histone H2AvD phosphoS137 Antibody
Application Note :
Histone H2AvD pS137 Antibody is tested in ELISA, Immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.1 mg/ml
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100
Default Unit :
µg
ELISA Dilution :
1:29,000 - 1:30,000
Immunohistochemistry Dilution :
2 ug/ml
Western Blot Dilution :
1:400 - 1:1,600
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Gene Name :
His2Av
Harmonization Code :
3002.14.0000
UNSPSC :
Antibodies
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Affinity purified Anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known.
Species Reactivity :
D. melanogaster
Tested Applications :
ELISA; IHC; WB
Suggested Applications :
ChIP; EM; IF; Multiplex
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-H2AvD pS137 antibody, rabbit anti-Histone H2A.v pS137 antibody, H2AvD protein antibody, H2A.F/Z, H2A.Z, H2AvD, His2AvD, His2Av
Target Species :
Drosophila melanogaster
Background :
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.
Immunogen Type :
Conjugated Peptide
Low Endotoxin :
No
Post Translational Modification :
Phosphorylation
Sample Size :
No
Target Domain of Reactivity :
C-Terminus
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2026 Price :
509.00 USD
UniProt :
P08985
NCBI :
17738227
Primary Image Name :
Immunohistochemistry
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.
browse more products
questions and comments