product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Beta Actin Antibody
catalog :
600-401-886
quantity :
200
price :
https://www.rockland.com/search/?searchString=600-401-886
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA
more info or order :
Rockland Immunochemicals product webpage
citations: 17
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:500; fig 6
Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, et al. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun. 2016;7:11876 pubmed publisher
  • western blot; human; fig 1
CARR F, Tai P, Barnum M, Gillis N, Evans K, Taber T, et al. Thyroid Hormone Receptor-? (TR?) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer. Endocrinology. 2016;157:3278-92 pubmed publisher
Rodrigues Amorim D, Iglesias Martínez Almeida M, Rivera Baltanas T, Fernández Palleiro P, Freiría Martínez L, Rodríguez Jamardo C, et al. The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia. Int J Mol Sci. 2021;22: pubmed publisher
Ili x107 N, Tao Y, Boutros Suleiman S, Kadali V, Emanuelli A, Levy Cohen G, et al. SMURF2-mediated ubiquitin signaling plays an essential role in the regulation of PARP1 PARylating activity, molecular interactions, and functions in mammalian cells. FASEB J. 2021;35:e21436 pubmed publisher
Rosas Ballina M, Guan X, Schmidt A, Bumann D. Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis. Front Immunol. 2020;11:131 pubmed publisher
Olive J, Qin Y, Decristo M, Laszewski T, Greathouse F, McAllister S. Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies. PLoS ONE. 2018;13:e0198790 pubmed publisher
Gillis N, Taber T, Bolf E, Beaudet C, Tomczak J, White J, et al. Thyroid Hormone Receptor ? Suppression of RUNX2 Is Mediated by Brahma-Related Gene 1-Dependent Chromatin Remodeling. Endocrinology. 2018;159:2484-2494 pubmed publisher
Borroni A, Emanuelli A, Shah P, Ilic N, Apel Sarid L, Paolini B, et al. Smurf2 regulates stability and the autophagic-lysosomal turnover of lamin A and its disease-associated form progerin. Aging Cell. 2018;17: pubmed publisher
Dai J, Luftig M. Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation. J Immunol. 2018;200:1727-1736 pubmed publisher
Coleman M, Ha S, Haeryfar S, Barr S, Kim S. Cathepsin B plays a key role in optimal production of the influenza A virus. J Virol Antivir Res. 2018;2018:1-20 pubmed publisher
Lodola F, Laforenza U, Cattaneo F, Ruffinatti F, Poletto V, Massa M, et al. VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells. Oncotarget. 2017;8:95223-95246 pubmed publisher
Chen X, Wang W, Man H, Li P, Shan B. Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice. Oncol Lett. 2017;13:2207-2215 pubmed publisher
Maragkoudaki M, Vaiopoulou A, Theodoropoulos G, Legaki E, Sechi L, Karamanolis G, et al. Specific detection of OCT4 isoforms in inflammatory bowel disease. Gut Pathog. 2015;7:25 pubmed publisher
Rae D, Hocum J, Bii V, Deeg H, Trobridge G. A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis. Oncotarget. 2015;6:30664-74 pubmed publisher
Koh D, Powell D, Blake S, Hoffman J, Hopkins M, Feng X. Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel. Oncol Rep. 2015;34:1589-98 pubmed publisher
Hopkins M, Feng X, Liu M, Parker L, Koh D. Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells. Int J Oncol. 2015;46:2267-76 pubmed publisher
Dragoni S, Turin I, Laforenza U, Potenza D, Bottino C, Glasnov T, et al. Store-operated Ca2+ entry does not control proliferation in primary cultures of human metastatic renal cellular carcinoma. Biomed Res Int. 2014;2014:739494 pubmed publisher
product information
Catalog Number :
600-401-886
Name :
Anti-Beta Actin (RABBIT) Antibody - 600-401-886
Display Name :
Beta Actin Antibody
Application Note :
Anti-beta Actin Antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
200
Default Unit :
µg
ELISA Dilution :
1:10,000 - 1:40,000
IF Microscopy Dilution :
1:500 - 1:2,000
Western Blot Dilution :
1:1,000 - 1:4,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Gene Name :
ACTB
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein.  A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Species Reactivity :
Human; Mouse; Leopard Frog
Tested Applications :
ELISA; IF; WB
Storage :
Store beta-Actin Loading Control Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
Target Species :
Human
Background :
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Immunogen Type :
Conjugated Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
C-Terminus
Application Text :
ELISA,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2024 Price :
$379.00
UniProt :
P60709
NCBI :
NP_001092.1
Primary Image Name :
Anti-Beta Actin Antibody - Immunohistochemistry
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.