Ready-To-Use VDAC/Porin Antibody | Rockland Immunochemicals 600-401-882-RTU product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Ready-To-Use VDAC/Porin Antibody

catalog :

600-401-882-RTU

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Western Blot Results of Ready to Use Rabbit Anti-VDAC/Porin Antibody. Lane 1: Opal Pre-stained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: HEK293T WC Lysate. Lane 3: HeLa WC Lysate (p/n W09-000-364). Load: 10µL. Primary Antibody: Ready to Use Rabbit Anti-VDAC1/Porin Antibody 1:1000 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30 min RT. Block: BlockOut Buffer (p/n MB-073). Expect: ~31 kDa.

product information

Catalog Number :

600-401-882-RTU

Name :

Ready-To-Use Anti-VDAC/Porin (RABBIT) Antibody - 600-401-882-RTU

Display Name :

Ready-To-Use VDAC/Porin Antibody

Application Note :

Ready to Use Anti-VDAC/Porin Antibody has been optimized and validated in western blot at 1:1000 dilution. This Anti-VDAC/Porin (RTU) Antibody is sufficient to run 10 western blots. Expect a band approximately 30-33kDa in size corresponding to VDAC/Porin by western blotting in appropriate cell lysate or extract. Although not tested, this antibody is likely functional in ELISA and Immunohistochemistry. Optimal titers for applications should be determined by the researcher.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

sufficient to run 10 immunoblot tests

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

User Optimized

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

VDAC1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.

Packing Type :

Wet Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

RTU Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).

Species Reactivity :

human

Stabilizer :

0.01% Bovine Serum Albumin (BSA), 25% (v/v) Glycerol

Storage :

Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.

Synonyms :

Loading Control Antibody, VDAC-1 Antibody, POR1 Antibody

Target Species :

Human

Background :

VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mito-chondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations. All Rockland Immunochemical’s VDAC/Porin antibodies are affinity-purified to assure both high affinity and specificity. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P21796

NCBI :

NP_003365.1

Primary Image Name :

Western Blot Results of RTU Rabbit Anti-VDAC/Porin Antibody.

more info or order :

Rockland Immunochemicals product webpage