Alpha-Tubulin Antibody | Rockland Immunochemicals 600-401-880 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Alpha-Tubulin Antibody

catalog :

600-401-880

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 10

Reference
Xiao H, Wyler E, Milek M, Grewe B, Kirchner P, Ekici A, et al. CRNKL1 Is a Highly Selective Regulator of Intron-Retaining HIV-1 and Cellular mRNAs. MBio. 2021;12: pubmed publisher
Garland W, Comet I, Wu M, Radzisheuskaya A, Rib L, Vitting Seerup K, et al. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. Cell Rep. 2019;29:1800-1811.e6 pubmed publisher
Lloret Llinares M, Karadoulama E, Chen Y, Wojenski L, Villafano G, Bornholdt J, et al. The RNA exosome contributes to gene expression regulation during stem cell differentiation. Nucleic Acids Res. 2018;46:11502-11513 pubmed publisher
Brown C, Dayan S, Wong S, Kaczmarek A, Hope C, Pederson S, et al. FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. Oncotarget. 2018;9:27708-27727 pubmed publisher
Kim J, Kim Y. Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3. Sci Rep. 2018;8:8680 pubmed publisher
Kapadia M, Bijelić D, Zhao H, Ma D, Stojanovich L, Milosevic M, et al. Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling. Acta Neuropathol Commun. 2017;5:70 pubmed publisher
Ghosh A, Abdelwahab S, Reeber S, Reidel B, Marklew A, Garrison A, et al. Little Cigars are More Toxic than Cigarettes and Uniquely Change the Airway Gene and Protein Expression. Sci Rep. 2017;7:46239 pubmed publisher
Liao H, Ren X, Peterson B, Marshak D, Yau K, Gamlin P, et al. Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations. J Comp Neurol. 2016;524:2845-72 pubmed publisher
Lloret Llinares M, Mapendano C, Martlev L, Lykke Andersen S, Jensen T. Relationships between PROMPT and gene expression. RNA Biol. 2016;13:6-14 pubmed publisher
Li J, Dou X, Li S, Zhang X, Zeng Y, Song Z. Nicotinamide ameliorates palmitate-induced ER stress in hepatocytes via cAMP/PKA/CREB pathway-dependent Sirt1 upregulation. Biochim Biophys Acta. 2015;1853:2929-36 pubmed publisher

product information

Catalog Number :

600-401-880

Name :

Anti-ALPHA-TUBULIN (RABBIT) Antibody - 600-401-880

Display Name :

Alpha-Tubulin Antibody

Application Note :

Anti-Tubulin Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.1mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

200 µg

Default Unit :

µg

ELISA Dilution :

1:90,000

Immunohistochemistry Dilution :

1:500 - 1:2,000

IF Microscopy Dilution :

1:500 - 1:2,000

Western Blot Dilution :

1:1,000 - 1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

TUBA1B

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

Species Reactivity :

human, mouse, rat

Storage :

Store Anti-Tubulin Loading Control Antibody at -20° C prior to opening. Aliquot Loading Control Antibody contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

Target Species :

Human

Background :

Tubulin Loading Control Antibody recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control. Anti-Alpha-tubulin antibody is ideal for investigators involved in cell cycle protein research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P68363

NCBI :

17986283

Primary Image Name :

Anti-Alpha Tubulin - Western Blot

more info or order :

Rockland Immunochemicals product webpage