ROBO-1 Antibody | Rockland Immunochemicals 600-401-692 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

ROBO-1 Antibody

catalog :

600-401-692

quantity :

100

price :

https://www.rockland.com/search/?searchString=600-401-692

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

citations: 2

Reference
Yusuf B, Mukovozov I, Patel S, Huang Y, Liu G, Reddy E, et al. The neurorepellent, Slit2, prevents macrophage lipid loading by inhibiting CD36-dependent binding and internalization of oxidized low-density lipoprotein. Sci Rep. 2021;11:3614 pubmed publisher
Pluthero F, Kahr W. Imaging Platelets and Megakaryocytes by High-Resolution Laser Fluorescence Microscopy. Methods Mol Biol. 2018;1812:13-31 pubmed publisher

product information

Catalog Number :

600-401-692

Name :

Anti-ROBO-1 (RABBIT) Antibody - 600-401-692

Display Name :

ROBO-1 Antibody

Application Note :

This affinity purified antibody has been tested for use in ELISA, western blot, and immunohistochemistry. It may be suitable for immunofluorescence and IP. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~181 kDa in size corresponding to ROBO-1 by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.98 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100

Default Unit :

µg

ELISA Dilution :

1:30,000 - 1:60,000

Immunohistochemistry Dilution :

2 µg/ml to 10 µg/ml

IF Microscopy Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:500 - 1:3,000

Expiration :

Expiration date is one (1) year from date of receipt.

Format :

IgG

Gene Name :

ROBO1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human ROBO-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO-1. Cross reactivity with ROBO-1 homologues from other sources has not been determined.

Species Reactivity :

Human; Mouse

Tested Applications :

ELISA; IHC; WB

Suggested Applications :

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-ROBO1 antibody, ROBO 1, ROBO-1, hROBO-1, Roundabout homolog 1, Deleted in U twenty twenty, DUTT1, DUTT-1

Target Species :

Human

Background :

ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2. The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein. ROBO-1 is a widely expressed protein with the exception of the kidney. Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.

Immunogen Type :

Conjugated Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2024 Price :

$475.00

UniProt :

Q2M1J3

NCBI :

AAI12337.1

Primary Image Name :

Immunohistochemistry - Anti-ROBO-1 Antibody

more info or order :

Rockland Immunochemicals product webpage