E2F-1 phospho S364 Antibody | Rockland Immunochemicals 600-401-690 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

E2F-1 phospho S364 Antibody

catalog :

600-401-690

quantity :

100

price :

449.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Reference
Metin Armağan D, Comunoglu N, Bulut G, Kadioglu P, Kameda H, Gazioglu N, et al. A Novel Expression Profile of Cell Cycle and DNA Repair Proteins in Nonfunctioning Pituitary Adenomas. Endocr Pathol. 2020;31:2-13 pubmed publisher

product information

Catalog Number :

600-401-690

Name :

Anti-E2F-1 pS364 (RABBIT) Antibody - 600-401-690

Display Name :

E2F-1 phospho S364 Antibody

Application Note :

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to phosphorylated E2F-1 by western blotting in the appropriate cell lysate or extract. Less than 0.5% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS364 of E2F-1.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100

Default Unit :

µg

ELISA Dilution :

1:20,000 - 1:100,000

Immunohistochemistry Dilution :

2 mg/ml - 20 µg/ml

Western Blot Dilution :

1:250 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

E2F1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human E2F-1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against the phosphorylated form of human E2F-1 at the pS364 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human E2F-1 pS364 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with E2F-1 homologues from other sources has not been determined.

Species Reactivity :

H. sapiens (Human); Mus musculus (Mouse)

Tested Applications :

ELISA; IHC; WB

Suggested Applications :

Other

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-E2F-1 pS364 Antibody, E2F 1 antibody, transcription factor E2F1 antibody, E2F1 antibody, E2F transcription factor 1 antibody, PBR 3 antibody, PBR3 antibody, Retinoblastoma binding protein 3, RBBP-3, pRB-binding protein E2F-1, Retinoblastoma-associated protein 1, RBAP-1

Target Species :

Human

Background :

E2F-1 (also known as transcription factor E2F-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains conserved through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and two other members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis as well as it can block adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Anti-E2F-1 pS364 Antibody is useful for researchers involved in transcription factor and DNA binding research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2023 Price :

449.00 USD

UniProt :

Q01094

NCBI :

1869

Primary Image Name :

Anti-E2F-1 pS364 Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage