product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
AURORA KINASE B phospho T232 Antibody
catalog :
600-401-677
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
more info or order :
Rockland Immunochemicals product webpage
citations: 10
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 3
Lee S, Jeong A, Park J, Han S, Jang C, Kim K, et al. IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells. Cell Mol Life Sci. 2016;73:3375-86 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 2f
Mo F, Zhuang X, Liu X, Yao P, Qin B, Su Z, et al. Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Nat Chem Biol. 2016;12:226-32 pubmed publisher
Novais Cruz M, Alba Abad M, van IJcken W, Galjart N, Jeyaprakash A, Maiato H, et al. Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription. elife. 2018;7: pubmed publisher
Drpic D, Almeida A, Aguiar P, Renda F, Damas J, Lewin H, et al. Chromosome Segregation Is Biased by Kinetochore Size. Curr Biol. 2018;28:1344-1356.e5 pubmed publisher
Skowyra A, Allan L, Saurin A, Clarke P. USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability. Cell Rep. 2018;23:852-865 pubmed publisher
Xie C, Lin X, Wu D, Tan Y, Cheng C, Zhang J. Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway. Oncotarget. 2018;9:13783-13795 pubmed publisher
Shrestha R, Conti D, Tamura N, Braun D, Ramalingam R, Cieśliński K, et al. Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells. Nat Commun. 2017;8:150 pubmed publisher
Redli P, Gasic I, Meraldi P, Nigg E, Santamaria A. The Ska complex promotes Aurora B activity to ensure chromosome biorientation. J Cell Biol. 2016;215:77-93 pubmed
Asghar A, Lajeunesse A, Dulla K, Combes G, Thebault P, Nigg E, et al. Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation. Nat Commun. 2015;6:8364 pubmed publisher
Nijenhuis W, Vallardi G, Teixeira A, Kops G, Saurin A. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 2014;16:1257-64 pubmed publisher
product information
Catalog Number :
600-401-677
Name :
Anti-Aurora B pT232 (RABBIT) Antibody - 600-401-677
Display Name :
AURORA KINASE B phospho T232 Antibody
Application Note :
Phospho pT232 Aurora B antibody has been tested for use in ELISA, immunohistochemistry, and by western blot.  See below for specific protocol.  Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract.  HeLa cell lysate can be used as a positive control.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
0.87 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:10,000 - 1:30,000
Immunohistochemistry Dilution :
User Optimized
IF Microscopy Dilution :
User Optimized
Western Blot Dilution :
1:250 - 1:2,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Gene Name :
AURKB
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein.  Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA.  No reaction is expected against Aurora Kinase A.  However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee.  Cross reactivity with Aurora Kinase B from other sources is not known.
Species Reactivity :
human, monkey
Storage :
Store Phospho Specific Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-Aurora B pT232 Antibody, Phospho Aurora B, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody
Target Species :
Human
Background :
Aurora Kinase B (Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Anti-AUROA B pT232 Antibody is useful for researchers interested in gene expression, DNA damage, cytokinesis, and transcription activities.
Immunogen Type :
Peptide
Low Endotoxin :
No
Post Translational Modification :
Phosphorylation
Sample Size :
No
Target Domain of Reactivity :
Internal
Application Text :
ELISA,Immunohistochemistry,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q96GD4
NCBI :
83776600
Primary Image Name :
Anti-Aurora B pT232 antibody - Western Blot
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.