PC2 Antibody | Rockland Immunochemicals 600-401-478 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PC2 Antibody

catalog :

600-401-478

quantity :

100 µg

price :

369.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

600-401-478

Name :

Anti-PC2 [RABBIT] Antibody - 600-401-478

Display Name :

PC2 Antibody

Application Note :

This affinity purified antibody has been tested for use in immunofluorescence staining of cultured cells and western blotting using transfected cell lysates. The antibody is also functional by ELISA. Reactivity in other immunoassays is unknown. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). In immunofluorescence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure below). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.36 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:40,000

IF Microscopy Dilution :

1:100 - 1:400

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:1,000 - 1:4,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CBX4

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 90-115 of Human PC2 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PC2 protein.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-PC2 antibody, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4

Target Species :

Human

Background :

PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to depression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,IF Microscopy,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

O00257

NCBI :

O00257.3

Primary Image Name :

Anti-hPC2 Antibody - Immunofluorescence Microscopy

more info or order :

Rockland Immunochemicals product webpage