Mcl-1 Antibody | Rockland Immunochemicals 600-401-394 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Mcl-1 Antibody

catalog :

600-401-394

quantity :

100 µg

price :

369.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3a western blot; mouse; loading ...; fig 1d	Haikala H, Anttila J, Marques E, Raatikainen T, Ilander M, Hakanen H, et al. Pharmacological reactivation of MYC-dependent apoptosis induces susceptibility to anti-PD-1 immunotherapy. Nat Commun. 2019;10:620 pubmed publisher
western blot; mouse; fig 3d	Myasnikov A, Kundhavai Natchiar S, Nebout M, Hazemann I, Imbert V, Khatter H, et al. Structure-function insights reveal the human ribosome as a cancer target for antibiotics. Nat Commun. 2016;7:12856 pubmed publisher
immunocytochemistry; mouse; 1:200; fig 7 western blot; mouse; fig 7	Thornton T, Delgado P, Chen L, Salas B, Krementsov D, Fernández M, et al. Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response. Nat Commun. 2016;7:10553 pubmed publisher
western blot; mouse; fig 9	Wang S, Ni H, Dorko K, Kumer S, Schmitt T, Nawabi A, et al. Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget. 2016;7:17681-98 pubmed publisher
western blot; mouse; fig 6	Sochalska M, Ottina E, Tuzlak S, Herzog S, Herold M, Villunger A. Conditional knockdown of BCL2A1 reveals rate-limiting roles in BCR-dependent B-cell survival. Cell Death Differ. 2016;23:628-39 pubmed publisher
western blot; mouse; loading ...; fig 1	Schuler F, Baumgartner F, Klepsch V, Chamson M, MÃ¼ller Holzner E, Watson C, et al. The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland. Cell Death Differ. 2016;23:41-51 pubmed publisher
	Suzuki T, Okamoto T, Katoh H, Sugiyama Y, Kusakabe S, Tokunaga M, et al. Infection with flaviviruses requires BCLXL for cell survival. PLoS Pathog. 2018;14:e1007299 pubmed publisher
	Guerra R, Bird G, Harvey E, Dharia N, Korshavn K, Prew M, et al. Precision Targeting of BFL-1/A1 and an ATM Co-dependency in Human Cancer. Cell Rep. 2018;24:3393-3403.e5 pubmed publisher
	Schubert F, Rapp J, Brauns Schubert P, Schlicher L, Stock K, Wissler M, et al. Requirement of GSK-3 for PUMA induction upon loss of pro-survival PI3K signaling. Cell Death Dis. 2018;9:470 pubmed publisher
	Girnius N, Davis R. JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins. Cell Rep. 2017;21:1910-1921 pubmed publisher
	Singh P, Roukounakis A, Frank D, Kirschnek S, Das K, Neumann S, et al. Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis. Genes Dev. 2017;31:1754-1769 pubmed publisher
	Tuzlak S, Schenk R, Vasanthakumar A, Preston S, Haschka M, Zotos D, et al. The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection. Cell Death Differ. 2017;24:523-533 pubmed publisher
	Sochalska M, Schuler F, Weiss J, Prchal Murphy M, Sexl V, Villunger A. MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis. Oncogene. 2017;36:2066-2073 pubmed publisher
	Goodall K, Finch Edmondson M, van Vuuren J, Yeoh G, Gentle I, Vince J, et al. Cycloheximide Can Induce Bax/Bak Dependent Myeloid Cell Death Independently of Multiple BH3-Only Proteins. PLoS ONE. 2016;11:e0164003 pubmed publisher
	Ohmer M, Weber A, Sutter G, Ehrhardt K, Zimmermann A, HÃ¤cker G. Anti-apoptotic Bcl-XL but not Mcl-1 contributes to protection against virus-induced apoptosis. Cell Death Dis. 2016;7:e2340 pubmed publisher
	Lee S, Wales T, Escudero S, Cohen D, Luccarelli J, Gallagher C, et al. Allosteric inhibition of antiapoptotic MCL-1. Nat Struct Mol Biol. 2016;23:600-7 pubmed publisher
	D Orsi B, Engel T, Pfeiffer S, Nandi S, Kaufmann T, Henshall D, et al. Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose Polymerase-Dependent Cell Death Pathways and Protects against Excitotoxic and Seizure-Induced Neuronal Injury. J Neurosci. 2016;36:4564-78 pubmed publisher
	Vier J, Groth M, Sochalska M, Kirschnek S. The anti-apoptotic Bcl-2 family protein A1/Bfl-1 regulates neutrophil survival and homeostasis and is controlled via PI3K and JAK/STAT signaling. Cell Death Dis. 2016;7:e2103 pubmed publisher
	Chen H, Kanai M, Inoue Yamauchi A, Tu H, Huang Y, Ren D, et al. An interconnected hierarchical model of cell death regulation by the BCL-2 family. Nat Cell Biol. 2015;17:1270-81 pubmed publisher
	Omari S, Waters M, Naranian T, Kim K, Perumalsamy A, Chi M, et al. Mcl-1 is a key regulator of the ovarian reserve. Cell Death Dis. 2015;6:e1755 pubmed publisher
	Williams J, Ni H, Haynes A, Manley S, Li Y, Jaeschke H, et al. Chronic Deletion and Acute Knockdown of Parkin Have Differential Responses to Acetaminophen-induced Mitophagy and Liver Injury in Mice. J Biol Chem. 2015;290:10934-46 pubmed publisher

product information

Catalog Number :

600-401-394

Name :

Anti-Mcl-1 (RABBIT) Antibody - 600-401-394

Display Name :

Mcl-1 Antibody

Application Note :

This antibody is suitable for western blotting, immunoprecipitation, and ELISA. This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence. Cross reactivity with Mcl-1 from other sources is unknown. This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size. The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.2mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Western Blot Dilution :

1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Mcl1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity-purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Species Reactivity :

Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody

Target Species :

Mouse

Background :

Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

P97287

NCBI :

P97287.3

Primary Image Name :

Anti-Mcl-1 Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage