DYKDDDDK Tag (Anti-FLAG®) Antibody | Rockland Immunochemicals 600-401-383 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

DYKDDDDK Tag (Anti-FLAG®) Antibody

catalog :

600-401-383

quantity :

250

price :

379.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:5000; loading ...; fig 3f	De Giorgi M, Li A, Hurley A, Barzi M, Doerfler A, Cherayil N, et al. Targeting the Apoa1 locus for liver-directed gene therapy. Mol Ther Methods Clin Dev. 2021;21:656-669 pubmed publisher
	H xe4 usl A, Bajaj T, Brix L, P xf6 hlmann M, Häfner K, De Angelis M, et al. Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism. Sci Adv. 2022;8:eabi4797 pubmed publisher
	Fusi L, Paudel R, Meder K, Schlosser A, Schrama D, Goebeler M, et al. Interaction of transcription factor FoxO3 with histone acetyltransferase complex subunit TRRAP modulates gene expression and apoptosis. J Biol Chem. 2022;298:101714 pubmed publisher
	Haines A, Wesolowski J, Ryan N, Monteiro Br xe1 s T, Paumet F. Cross Talk between ARF1 and RhoA Coordinates the Formation of Cytoskeletal Scaffolds during Chlamydia Infection. MBio. 2021;12:e0239721 pubmed publisher
	Herrera Cruz M, Yap M, Tahbaz N, Phillips K, Thomas L, Thomas G, et al. Rab32 uses its effector reticulon 3L to trigger autophagic degradation of mitochondria-associated membrane (MAM) proteins. Biol Direct. 2021;16:22 pubmed publisher
	Martinelli S, Anderzhanova E, Bajaj T, Wiechmann S, Dethloff F, Weckmann K, et al. Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion. Nat Commun. 2021;12:4643 pubmed publisher
	Nabar N, Heijjer C, Shi C, Hwang I, Ganesan S, Karlsson M, et al. LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells. Autophagy. 2021;:1-19 pubmed publisher
	Li Z, Gu S, Quan Y, Varadaraj K, Jiang J. Development of a potent embryonic chick lens model for studying congenital cataracts in vivo. Commun Biol. 2021;4:325 pubmed publisher
	Carr H, Chang J, Frost J. The PDZ Domain Protein SYNJ2BP Regulates GRK-Dependent Sst2A Phosphorylation and Downstream MAPK Signaling. Endocrinology. 2021;162: pubmed publisher
	Chang C, Xu X, Li M, Xin D, Ding L, Wang Y, et al. Pathogenic mutations reveal a role of RECQ4 in mitochondrial RNA:DNA hybrid formation and resolution. Sci Rep. 2020;10:17033 pubmed publisher
	Lindner F, Milne Davies B, Langenfeld K, Stiewe T, Diepold A. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun. 2020;11:2381 pubmed publisher
	Sadek J, Wuo M, Rooklin D, Hauenstein A, Hong S, Gautam A, et al. Modulation of virus-induced NF-κB signaling by NEMO coiled coil mimics. Nat Commun. 2020;11:1786 pubmed publisher
	Skotnicka D, Steinchen W, Szadkowski D, Cadby I, Lovering A, Bange G, et al. CdbA is a DNA-binding protein and c-di-GMP receptor important for nucleoid organization and segregation in Myxococcus xanthus. Nat Commun. 2020;11:1791 pubmed publisher
	Zannas A, Jia M, Häfner K, Baumert J, Wiechmann T, Pape J, et al. Epigenetic upregulation of FKBP5 by aging and stress contributes to NF-κB-driven inflammation and cardiovascular risk. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
	Olsen C, Memarzadeh K, Ulu A, Carr H, Bean A, Frost J. Regulation of Somatostatin Receptor 2 Trafficking by C-Tail Motifs and the Retromer. Endocrinology. 2019;160:1031-1043 pubmed publisher
	Terra L, Wailemann R, Dos Santos A, Gomes V, Silva R, Laporte A, et al. Heat shock protein B1 is a key mediator of prolactin-induced beta-cell cytoprotection against oxidative stress. Free Radic Biol Med. 2019;134:394-405 pubmed publisher
	Yue Y, Nabar N, Shi C, Kamenyeva O, Xiao X, Hwang I, et al. SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death. Cell Death Dis. 2018;9:904 pubmed publisher
	Shimo T, Tachibana K, Obika S. Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening. PLoS ONE. 2018;13:e0197373 pubmed publisher
	Geng S, Miyagi A, Umen J. Evolutionary divergence of the sex-determining gene MID uncoupled from the transition to anisogamy in volvocine algae. Development. 2018;145: pubmed publisher
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	Hu Z, Shi W, Riquelme M, Shi Q, Biswas S, Lo W, et al. Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation. Sci Rep. 2017;7:5298 pubmed publisher
	Smith T, Li J, Dores M, Trejo J. Protease-activated receptor-4 and purinergic receptor P2Y12 dimerize, co-internalize, and activate Akt signaling via endosomal recruitment of β-arrestin. J Biol Chem. 2017;292:13867-13878 pubmed publisher
	Smith T, Coronel L, Li J, Dores M, Nieman M, Trejo J. Protease-activated Receptor-4 Signaling and Trafficking Is Regulated by the Clathrin Adaptor Protein Complex-2 Independent of ?-Arrestins. J Biol Chem. 2016;291:18453-64 pubmed publisher
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	Gassen N, Hartmann J, Zannas A, Kretzschmar A, Zschocke J, Maccarrone G, et al. FKBP51 inhibits GSK3Î² and augments the effects of distinct psychotropic medications. Mol Psychiatry. 2016;21:277-89 pubmed publisher

product information

Catalog Number :

600-401-383

Name :

Antibody for the detection of FLAG® conjugated proteins (RABBIT) - 600-401-383

Display Name :

DYKDDDDK Tag (Anti-FLAG®) Antibody

Application Note :

This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAGä containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. Rockland Immunochemical's polyclonal antibody to detect FLAG™ conjugated proteins binds FLAG™ containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.10

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

250

Default Unit :

µg

ELISA Dilution :

1:20,000 - 1:40,000

Immunohistochemistry Dilution :

User Optimized

Western Blot Dilution :

1:2,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of receipt.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. This antibody reacts with FLAG™ conjugated proteins.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays. This polyclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

Species Reactivity :

FLAG-Tag

Tested Applications :

ELISA; WB

Suggested Applications :

IF; IHC; IP; Multiplex

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit antibody for the detection of FLAG™ conjugated proteins, rabbit anti DYKDDDDK

Background :

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.

Immunogen Type :

Conjugated Peptide

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2025 Price :

379.00 USD

Primary Image Name :

FLAG™ conjugated proteins - Western Blot

more info or order :

Rockland Immunochemicals product webpage