product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
RFP Antibody Pre-adsorbed
catalog :
600-401-379
quantity :
100
price :
345.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 1093
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:200; loading ...; fig 4i
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  • immunohistochemistry - paraffin section; mouse; 1:250; loading ...; fig 5b
Chung M, Bujnis M, Barkauskas C, Kobayashi Y, Hogan B. Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation. Development. 2018;145: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig s1a
Chen Z, Donnelly C, Dominguez B, Harada Y, Lin W, Halim A, et al. p75 Is Required for the Establishment of Postnatal Sensory Neuron Diversity by Potentiating Ret Signaling. Cell Rep. 2017;21:707-720 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; fig s1e
Lim J, Ibaseta A, Fischer M, Cancilla B, O Young G, Cristea S, et al. Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer. Nature. 2017;545:360-364 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig s5a
Tang A, Choi J, Kotzin J, Yang Y, Hong C, Hobson N, et al. Endothelial TLR4 and the microbiome drive cerebral cavernous malformations. Nature. 2017;545:305-310 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...
Ramjee V, Li D, Manderfield L, Liu F, Engleka K, Aghajanian H, et al. Epicardial YAP/TAZ orchestrate an immunosuppressive response following myocardial infarction. J Clin Invest. 2017;127:899-911 pubmed publisher
  • western blot; mouse; 1:2000
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  • immunohistochemistry; mouse; 1:1000; fig 1
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product information
Catalog Number :
600-401-379
Name :
Anti-RFP (RABBIT) Antibody (Min X Hu Ms & Rt Serum Proteins) - 600-401-379
Display Name :
RFP Antibody Pre-adsorbed
Application Note :
RFP Antibody Pre-adsorbed is designed for detecting RFP and its variants, tested across ELISA, Western blot, IF, IHC, and suitable for EM, FC, FISH, IP, and multiplex assays. It is applicable for ELISA in both sandwich and capture formats. Researchers should determine optimal titers for specific applications. Recommended starting dilutions are: ELISA 1:28,700 - 1:48,700; FC, IF, IHC 1:200 - 1:2,000; WB 1:1,000 - 1:5,000.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.03 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100
Default Unit :
µg
ELISA Dilution :
1:28,700 - 1:48,700
Flow Cytometry Dilution :
1:200 - 1:2,000
Immunohistochemistry Dilution :
1:200 - 1:2,000
IF Microscopy Dilution :
1:200 - 1:2,000
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
1:1,000 - 1:5,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Gene Name :
DsRed
Harmonization Code :
3002.14.0000
UNSPSC :
Antibodies
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma).  No reaction was observed against Human, Mouse or Rat serum proteins.
Species Reactivity :
RFP; mScarlet; rRFP; tdTomato
Tested Applications :
ELISA; IF; IHC; WB
Suggested Applications :
EM; FC; FISH; IP; Other
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
Background :
RFP Antibody Pre-adsorbed from Rockland Immunochemicals is a high-quality, rabbit polyclonal antibody specifically designed for the detection of red fluorescent protein (RFP) and its variants like mCherry, mScarlet, and tdTomato. This product is suitable for a broad range of applications including Western blot (WB), ELISA, immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and more. It is uniquely pre-adsorbed to minimize cross-reactivity, offering high specificity and sensitivity. With over 900 references, this product stands as a proven tool in the scientific community, supporting a wide range of research studies from cellular signaling to disease pathology.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
2026 Price :
345.00 USD
UniProt :
Q9U6Y8
Primary Image Name :
Immunofluorescence Microscopy
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.