V5 Epitope Tag Antibody | Rockland Immunochemicals 600-401-378 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

V5 Epitope Tag Antibody

catalog :

600-401-378

quantity :

100 µg

price :

243.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 3

Reference
Schweiger P, Clement D, Page M, Schepeler T, Zou X, Sirokmány G, et al. Lrig1 marks a population of gastric epithelial cells capable of long-term tissue maintenance and growth in vitro. Sci Rep. 2018;8:15255 pubmed publisher
Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J. 2018;37: pubmed publisher
Watanabe S, Simon V, Durham N, Kemp B, Machihara S, Kemal K, et al. The HIV-1 late domain-2 S40A polymorphism in antiretroviral (or ART)-exposed individuals influences protease inhibitor susceptibility. Retrovirology. 2016;13:64 pubmed publisher

product information

Catalog Number :

600-401-378

Name :

Anti-V5 EPITOPE TAG (RABBIT) Antibody - 600-401-378

Display Name :

V5 Epitope Tag Antibody

Application Note :

Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:60,000

Immunohistochemistry Dilution :

1:500 - 1:3,000

Western Blot Dilution :

1:5,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity-purified antibody is directed against V5 motif and is useful in determining its presence in various assays. This polyclonal anti-V5-tag antibody detects over-expressed proteins containing the V5 epitope tag. To date this antibody has reacted with all V5 tagged proteins tested so far. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. The antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to either the carboxy- terminal end of targeted proteins in transfected or transformed cells. Although not yet tested, expect reactivity with recombinant proteins prepared with the V5-epitope tag fused to the amino terminal end as well.

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Rabbit Anti-V5 Epitope Tag Antibody, Rabbit Anti V5 Epitope Tag Antibody, Rabbit Anti-V5 Tag

Background :

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG, HA and V5. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

Epitope Tag Type :

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

GFP and Epitopes

Conjugation Name :

Unconjugated

2020 Price :

243.00 USD

NCBI :

55775699

Primary Image Name :

Anti-V5 Epitipe Tag Polyclonal Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage