antibody

Rad9 phospho S1129 Antibody

600-401-345

100 µg

polyclonal

domestic rabbit

nonconjugated

western blot, ELISA

Anti-Rad9 pS1129 Yeast (RABBIT) Antibody - 600-401-345

Rad9 phospho S1129 Antibody

This phospho specific polyclonal antibody was tested by ELISA.  Data from ELISA indicates the antibody is reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. No reactivity is expected against the human or mouse analogs of RAD9.  Reactivity against RAD9 from other sources is unknown.   Although not tested, this antibody is likely functional by WB, IHC and IP. This product has been assayed against 0.1 µg of phosphorylated peptide (pS1129) in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) code # TMBE-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 is suggested for this product.  Minimal reactivity was detected against the non-phosphorylated form (S1129) of the immunizing peptide. This antibody appears to be specific for the active form (phosphorylated) of the protein.  Researchers should determine optimal titers for other applications.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

0.43 mg/mL

by UV absorbance at 280 nm

(None)

100 µg

µg

1:10,000 - 1:50,000

1:500- 1:2,000

Expiration date is one (1) year from date of opening.

IgG

RAD9

Rabbit

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1120-1145 from the aa1309 yeast Rad9 protein conjugated to KLH.

Dry Ice

Liquid (sterile filtered)

0.01% (w/v) Sodium Azide

This affinity purified antibody is directed against the phosphorylated form of yeast Rad9 at the pS1129 residue.  The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was purified against the immunizing peptide. This phosphor polyclonal antibody reacts with the phosphorylated Rad9 pS1129 and minimally with non-phosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown.

Saccharomyces cerevisiae

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Rabbit anti-Rad9 phospho antibody, Rabbit anti-Rad9 pS1129 antibody, Rad 9, Rad-9, Cell cycle checkpoint control protein antibody, Cell cycle checkpoint control protein RAD9A antibody, DNA repair exonuclease rad9 homolog A antibody, DNA repair protein RAD9, YDR217C, YD9934.02C

Yeast

Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9.  Cells have evolved multiple strategies for tolerating genomic damage. The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions.  Another cellular strategy is to delay cell-cycle transitions at multiple points.  The genetic control of these delays, termed `checkpoints', was first established in budding yeast where it was shown that the RAD9 gene functions in G 2 /M arrest after irradiation with X-rays.  Subsequently, it has become clear that Rad9 also functions at the G 1 /S, intra-S and mid-anaphase checkpoints.  Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation. Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR).  Rad9 may also have a pro-apoptotic function.  This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells.  Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphosphorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate. Mature yeast Rad9 is reported to have an apparent molecular weight of ~148kDa.  The human homolog is reported at 48.5 kDa.

Peptide

No

Phosphorylation

No

Internal

ELISA,Western Blot,

User Optimized

Primary Antibodies

Unconjugated

P14737

NP_010503.1

Summary Table.