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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Phosphotyrosine Antibody
catalog :
600-401-262
quantity :
100 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
600-401-262
Name :
Anti-PhosphoTyrosine (pY) (RABBIT) Antibody - 600-401-262
Display Name :
Phosphotyrosine Antibody
Application Note :
Anti-PhosphoTyrosine (pY) Antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays.  Anti-PhosphoTyrosine (pY) Antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. Anti-PhosphoTyrosine (pY) Antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, Anti-PhosphoTyrosine (pY) Antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
1:1,000- 1:5,000
Immunohistochemistry Dilution :
1:50
Immunoprecipitation Dilution :
10.0 µg/ 500 µg sample
Western Blot Dilution :
1:500
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This Anti-PhosphoTyrosine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-PhosphoTyrosine (pY) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.  Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphothreonine or phosphoserine.
Species Reactivity :
phosphotyrosine
Storage :
Store PhosphoTyrosine antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit
Background :
Phosphotyrosine is the tyrosine amino acid that has been phosphorylated and is considered one of the integral steps in signal transduction and general enzyme activity. Tyrosine is phosphorylated through protein kinases and through phosphorylation, phosphotyrosine can bind other proteins causing binding of multi-protein complexes that connect the upstream kinases and downstream effectors. Phosphotyrosine-binding domains, or PTB's, are pervasive throughout the study of signal transduction and disease. Anti-Phosphotyrosine (pY) Antibody is ideal for investigators in Cancer, Signal Transduction, and Cell Signaling research.
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.