antibody

Collagen Type I Antibody

600-401-103-0.5

500

https://www.rockland.com/search/?searchString=600-401-103-0.5

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat, bovine

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

Anti-Collagen Type I (RABBIT) Antibody - 600-401-103-0.5

Collagen Type I Antibody

Anti-Collagen Type I has been tested by Western blot, dot blot, and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, IP, native PAGE, immunofluorescence, and FC for highly sensitive qualitative analysis.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

1.00 mg/mL

by UV absorbance at 280 nm

(None)

500

µg

1:5,000 - 1:50,000

1:100

User Optimized

1:50 - 1:200

User Optimized

1:100

1:1,000 - 1:10,000

Expiration date is one (1) year from date of receipt.

IgG

COL1A1/A2

Rabbit

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Collagen Type I from human and bovine placenta

Wet Ice

Liquid (sterile filtered)

0.01% (w/v) Sodium Azide

COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Human; Mouse; Rat; Bovine; Pig

Dot Blot; IHC; WB

ELISA; FC; IF; IP; Multiplex

Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1, Collagen type I alpha 2 antibody

Mammalian

Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Collagen Type I is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera). Collagen Type I triple helix comprises of two alpha1 chains and one alpha2 chain. COL1A1/A2 could be useful for detecting melanoma, lung, liver, glioma, skin, stomach, and other cancers. Mutations in the gene may be related to caffey disease, osteogenesis, and ehlers-danlos syndrome. Anti-Collagen Type I Antibody is ideal for investigators involved in extracellular matrix protein, osteoporosis research, Cell Biology, Signal Transduction, and Stem Cell research.

Native Protein

No

No

ELISA,FLISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

User Optimized

Primary Antibodies

Unconjugated

$949.00

P02452

NP_000079.2

Anti-Collagen I Antibody - Immunohistochemistry