GFP Monoclonal Antibody | Rockland Immunochemicals 600-301-215M product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

GFP Monoclonal Antibody

catalog :

600-301-215M

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9F9.F9

application :

western blot, ELISA, immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

citations: 3

Reference
Kim J, Cooper J. Septins regulate junctional integrity of endothelial monolayers. Mol Biol Cell. 2018;29:1693-1703 pubmed publisher
Khlghatyan J, Evstratova A, Chamberland S, Marakhovskaia A, Bahremand A, Toth K, et al. Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3Î² Are Modulators of Anxiety and Glutamatergic Neurotransmission. Front Mol Neurosci. 2018;11:119 pubmed publisher
Terrell J, Wu H, Tsao C, Barber N, Servinsky M, Payne G, et al. Nano-guided cell networks as conveyors of molecular communication. Nat Commun. 2015;6:8500 pubmed publisher

product information

Catalog Number :

600-301-215M

Name :

Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215M

Display Name :

GFP Monoclonal Antibody

Application Note :

Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, FACS, and WB. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

9F9.F9

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:80,000 - 1:500,000

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

1:1,000 - 1:5,000

IF Microscopy Dilution :

User Optimized

Western Blot Dilution :

1:3000 - 1:30,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Host Animal :

Mouse

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.

Species Reactivity :

wt, rGFP, eGFP

Storage :

Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG1 kappa

Synonyms :

mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background :

Mouse anti-GFP antibody is functional by western blot, ELISA, Immunofluorescene Micorscopy and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

Epitope Tag Type :

GFP

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

GFP and Epitopes

Conjugation Name :

Unconjugated

Primary Image Name :

Western blot of Mouse Anti-GFP Antibody

more info or order :

Rockland Immunochemicals product webpage