product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
GFP Antibody Dylight™ 488 Conjugated Pre-Adsorbed
catalog :
600-141-215
quantity :
100
price :
https://www.rockland.com/search/?searchString=600-141-215
clonality :
polyclonal
host :
domestic goat
conjugate :
DyLight488
application :
western blot, ELISA
citations: 14
Reference
Lee P, Zhao D, Wong V, Chrysostomou V, Crowston J, Bui B. The Effect of Aging on Retinal Function and Retinal Ganglion Cell Morphology Following Intraocular Pressure Elevation. Front Aging Neurosci. 2022;14:859265 pubmed publisher
Paterson N, Lämmermann T. Macrophage network dynamics depend on haptokinesis for optimal local surveillance. elife. 2022;11: pubmed publisher
Bhargava A, Williart A, Maurin M, Davidson P, Jouve M, Piel M, et al. Inhibition of HIV infection by structural proteins of the inner nuclear membrane is associated with reduced chromatin dynamics. Cell Rep. 2021;36:109763 pubmed publisher
Freter R, Falletta P, Omrani O, Rasa M, Herbert K, Annunziata F, et al. Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells. Nat Commun. 2021;12:3318 pubmed publisher
Kim H, Jiang P. Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin. STAR Protoc. 2021;2:100443 pubmed publisher
Wang A, Wong V, Lee P, Bui B, Dudczig S, Vessey K, et al. Retinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout. Sci Rep. 2021;11:4184 pubmed publisher
Kolter J, Feuerstein R, Zeis P, Hagemeyer N, Paterson N, d Errico P, et al. A Subset of Skin Macrophages Contributes to the Surveillance and Regeneration of Local Nerves. Immunity. 2019;50:1482-1497.e7 pubmed publisher
Xu R, Brawner A, Li S, Liu J, Kim H, Xue H, et al. OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome. Cell Stem Cell. 2019;: pubmed publisher
Gentili M, Lahaye X, Nadalin F, Nader G, Puig Lombardi E, Hervé S, et al. The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus. Cell Rep. 2019;26:2377-2393.e13 pubmed publisher
Haas D, Meiler A, Geiger K, Vogt C, Preuss E, Kochs G, et al. Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity. PLoS Pathog. 2018;14:e1006980 pubmed publisher
Russo M, Latour L, McGavern D. Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury. Nat Immunol. 2018;19:442-452 pubmed publisher
Fletcher E, Greferath U, Saha S, Anderson E, Vessey K. Ganglion Cell Assessment in Rodents with Retinal Degeneration. Methods Mol Biol. 2018;1753:261-273 pubmed publisher
Begolly S, Olschowka J, Love T, Williams J, O Banion M. Fractionation enhances acute oligodendrocyte progenitor cell radiation sensitivity and leads to long term depletion. Glia. 2018;66:846-861 pubmed publisher
Bongianino R, Denegri M, Mazzanti A, Lodola F, Vollero A, Boncompagni S, et al. Allele-Specific Silencing of Mutant mRNA Rescues Ultrastructural and Arrhythmic Phenotype in Mice Carriers of the R4496C Mutation in the Ryanodine Receptor Gene (RYR2). Circ Res. 2017;121:525-536 pubmed publisher
product information
Catalog Number :
600-141-215
Name :
Anti-GFP (GOAT) Antibody DyLight™ 488 Conjugated Min X Hu Ms and Rt Serum Proteins - 600-141-215
Display Name :
GFP Antibody Dylight™ 488 Conjugated Pre-Adsorbed
Application Note :
Anti-GFP Dylight™ 488 Conjugated Antibody has been tested by dot blot and western blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
DL488
Size :
100
Default Unit :
µg
ELISA Dilution :
1:20,000 - 1:40,000
FLISA Dilution :
>1:10,000
Fluorochrome Protein Value :
5.7
IF Microscopy Dilution :
>1:5,000
Western Blot Dilution :
1:10,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
GFP Dylight™ 488 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse or Rat serum proteins.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
100 µL
Species Reactivity :
GFP; eGFP; rGFP
Tested Applications :
Dot Blot; WB
Suggested Applications :
FC; IF; IHC; IP; Multiplex
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store Conjugated anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
goat anti-GFP Antibody DyLight™ 488 Conjugation, DyLight™ 488 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Background :
GFP Dylight™ 488 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,FLISA,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
DyLight™ 488
2024 Price :
$449.00
UniProt :
P42212
Primary Image Name :
Western Blot of Goat Anti-GFP Antibody DyLight™ 488 MX3
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.