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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Biotin Antibody Phycoerythrin Conjugated
catalog :
600-108-098
quantity :
1 mL
clonality :
polyclonal
host :
domestic goat
conjugate :
RPE
application :
western blot, ELISA, immunohistochemistry, flow cytometry
more info or order :
product information
Catalog Number :
600-108-098
Name :
Anti-BIOTIN (GOAT) Antibody Phycoerythrin Conjugated - 600-108-098
Display Name :
Biotin Antibody Phycoerythrin Conjugated
Application Note :
Anti-Biotin detects biotin and biocytin by ELISA, Immunohistochemistry (IHC), and the antibody also recognizes biotin conjugated to various immunoglobulins. The antibody may be used in amplification techniques, immunoblotting, in situ nucleic acid hybridization, flow cytometry or Immunofluorescence microscopy.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
0.5 mg/mL
Concentration Definition :
by absorbance = 82.0 at 565 nm
Conjugation :
RPE
Size :
1 mL
Default Unit :
mL
ELISA Dilution :
1:50-1:200
Flow Cytometry Dilution :
1.0 µg/1,000,000 cells
Immunohistochemistry Dilution :
User Optimized
IF Microscopy Dilution :
1:50-1:200
Western Blot Dilution :
1:50-1:200
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Packing Type :
Wet Ice
Physical State :
Lyophilized
Preservative :
0.05% (w/v) Sodium Azide
Purity and Specificity :
Anti-Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Phycoerythrin, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
Reconstitution Volume :
1.0 mL
Species Reactivity :
Biotin
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Synonyms :
goat anti-Biotin Antibody PE Conjugation, Phycoerythrin conjugated goat anti-Biotin Antibody, Vitamin B4 antibody
Background :
Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Phycoerythrin Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
ABS Reagent Type :
Biotin
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Western Blot,
Category :
GFP and Epitopes
Conjugation Name :
R-Phycoerythrin (RPE)
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.
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