product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
GFP (GOAT) Antibody Biotin Conjugated
catalog :
600-106-215
quantity :
1
price :
https://www.rockland.com/search/?searchString=600-106-215
clonality :
polyclonal
host :
domestic goat
conjugate :
biotin
application :
western blot, ELISA, immunohistochemistry
more info or order :
Rockland Immunochemicals product webpage
citations: 28
Reference
Jones T, Naslavsky N, Caplan S. Differential requirements for the Eps15 homology domain proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic. 2022;23:360-373 pubmed publisher
Tran N, Carter S, De Mazière A, Ashkenazi A, Klumperman J, Walter P, et al. The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes. Science. 2021;374:52-57 pubmed publisher
Wieghofer P, Hagemeyer N, Sankowski R, Schlecht A, Staszewski O, Amann L, et al. Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling. EMBO J. 2021;40:e105123 pubmed publisher
Oorschot V, Lindsey B, Kaslin J, Ramm G. TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages. Sci Rep. 2021;11:899 pubmed publisher
Zulkefli K, Mahmoud I, Williamson N, Gosavi P, Houghton F, Gleeson P. A role for Rab30 in retrograde trafficking and maintenance of endosome-TGN organization. Exp Cell Res. 2021;399:112442 pubmed publisher
Li H, von Wangenheim D, Zhang X, Tan S, Darwish Miranda N, Naramoto S, et al. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytol. 2021;229:351-369 pubmed publisher
Cabukusta B, Berlin I, van Elsland D, Forkink I, Spits M, de Jong A, et al. Human VAPome Analysis Reveals MOSPD1 and MOSPD3 as Membrane Contact Site Proteins Interacting with FFAT-Related FFNT Motifs. Cell Rep. 2020;33:108475 pubmed publisher
Bohlen M, McCown T, Powell S, El Nahal H, Daw T, Basso M, et al. Adeno-Associated Virus Capsid-Promoter Interactions in the Brain Translate from Rat to the Nonhuman Primate. Hum Gene Ther. 2020;31:1155-1168 pubmed publisher
Cushnie A, El Nahal H, Bohlen M, May P, Basso M, Grimaldi P, et al. Using rAAV2-retro in rhesus macaques: Promise and caveats for circuit manipulation. J Neurosci Methods. 2020;345:108859 pubmed publisher
Jongsma M, Bakker J, Cabukusta B, Liv N, van Elsland D, Fermie J, et al. SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport. EMBO J. 2020;39:e102301 pubmed publisher
Andres Alonso M, Ammar M, Butnaru I, Gomes G, Acuña Sanhueza G, Raman R, et al. SIPA1L2 controls trafficking and local signaling of TrkB-containing amphisomes at presynaptic terminals. Nat Commun. 2019;10:5448 pubmed publisher
Bohlen M, El Nahal H, Sommer M. Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques. Front Neuroanat. 2019;13:84 pubmed publisher
Koerver L, Papadopoulos C, Liu B, Kravic B, Rota G, Brecht L, et al. The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage. EMBO Rep. 2019;20:e48014 pubmed publisher
Jangphattananont N, Sato H, Imamura R, Sakai K, Terakado Y, Murakami K, et al. Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach. Int J Mol Sci. 2019;20: pubmed publisher
Lee J, Kim M, Thomas S, Oorschot V, Ramm G, Mun Aui P, et al. Limiting neuronal nogo receptor 1 signaling during experimental autoimmune encephalomyelitis (EAE) preserves axonal transport and abrogates inflammatory demyelination. J Neurosci. 2019;: pubmed publisher
Yousefi O, Gunther M, Hörner M, Chalupsky J, Wess M, Brandl S, et al. Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. elife. 2019;8: pubmed publisher
Croop B, Han K. Facile single-molecule pull-down assay for analysis of endogenous proteins. Phys Biol. 2019;16:035002 pubmed publisher
Zöller T, Schneider A, Kleimeyer C, Masuda T, Potru P, Pfeifer D, et al. Silencing of TGFβ signalling in microglia results in impaired homeostasis. Nat Commun. 2018;9:4011 pubmed publisher
Maeder C, Kim J, Liang X, Kaganovsky K, Shen A, Li Q, et al. The THO Complex Coordinates Transcripts for Synapse Development and Dopamine Neuron Survival. Cell. 2018;174:1436-1449.e20 pubmed publisher
Liebmann T, Fritz N, Kruusmägi M, Westin L, Bernhem K, Bondar A, et al. Regulation of Neuronal Na,K-ATPase by Extracellular Scaffolding Proteins. Int J Mol Sci. 2018;19: pubmed publisher
Bender J, Bognar S, Camagna M, Donauer J, Eble J, Emig R, et al. Multiplexed antibody detection from blood sera by immobilization of in vitro expressed antigens and label-free readout via imaging reflectometric interferometry (iRIf). Biosens Bioelectron. 2018;115:97-103 pubmed publisher
McArthur K, Whitehead L, Heddleston J, Li L, Padman B, Oorschot V, et al. BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis. Science. 2018;359: pubmed publisher
Ge Y, Kang Y, Cassidy R, Moon K, Lewis R, Wong R, et al. Clptm1 Limits Forward Trafficking of GABAA Receptors to Scale Inhibitory Synaptic Strength. Neuron. 2018;97:596-610.e8 pubmed publisher
Bower N, Koltowska K, Pichol Thievend C, Virshup I, Paterson S, Lagendijk A, et al. Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish. Nat Neurosci. 2017;20:774-783 pubmed publisher
Goldmann T, Wieghofer P, Jordão M, Prutek F, Hagemeyer N, Frenzel K, et al. Origin, fate and dynamics of macrophages at central nervous system interfaces. Nat Immunol. 2016;17:797-805 pubmed publisher
De Henau S, Tilleman L, Vangheel M, Luyckx E, Trashin S, Pauwels M, et al. A redox signalling globin is essential for reproduction in Caenorhabditis elegans. Nat Commun. 2015;6:8782 pubmed publisher
Baek S, Copeland B, Yun E, Kwon S, Guemez Gamboa A, Schaffer A, et al. An AKT3-FOXG1-reelin network underlies defective migration in human focal malformations of cortical development. Nat Med. 2015;21:1445-54 pubmed publisher
Sztal T, Zhao M, Williams C, Oorschot V, Parslow A, Giousoh A, et al. Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function. Acta Neuropathol. 2015;130:389-406 pubmed publisher
product information
Catalog Number :
600-106-215
Name :
Anti-GFP (GOAT) Antibody Biotin Conjugated - 600-106-215
Display Name :
GFP (GOAT) Antibody Biotin Conjugated
Application Note :
Anti-GFP Biotin Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
BAC
Size :
1
Default Unit :
mg
ELISA Dilution :
1:50,000 - 1:80,000
Immunohistochemistry Dilution :
1:1,000 - 1:5,000
Western Blot Dilution :
1:2,000 - 1:10,000
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.  No reaction was observed against Human, Mouse and Rat Serum Proteins.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
1.0 mL
Species Reactivity :
GFP; eGFP; rGFP
Tested Applications :
ELISA; WB
Suggested Applications :
FC; IF; IHC; IP; Multiplex; Other; Purification
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store Anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
goat anti-GFP Antibody biotin Conjugation, biotin conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Background :
Conjugated Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Biotin
2024 Price :
$649.00
UniProt :
P42212
Primary Image Name :
ELISA Results of Goat Anti-GFP Antibody
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.