hCG Alpha chain Antibody | Rockland Immunochemicals 600-101-MR6S product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

hCG Alpha chain Antibody

catalog :

600-101-MR6S

quantity :

25 µL

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Western Blot of Goat Anti-Human Chorionic Gonadotropin Alpha Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: alpha hCG (0.05µg) [+]. Lane 3: alpha hCG/U-20S WCL (p/n W09-001-374) (0.05µg/20µg) [+]. Lane 4: U-20S WCL (p/n W09-001-374) (20µg) [-]. Lane 5: HeLa WCL (p/n W09-000-364) (35µg) [+]. Primary Antibody: Anti-α-hCG at 1:1000 overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG mx8 HRP Conjugated (p/n 605-703-125-0025) at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073). Exposure: 30 sec. Predicted MW: alpha subunit ~14.9kDa, full hCG ~37kDa, luteinizing hormone (LH) ~29kDa, thyroid stimulating hormone (TSH) ~28kDa, follicle stimulating hormone (FSH) ~30kDa. Observed MW: alpha subunit ~15-20kDa, full hCG ~35kDa.

product information

Catalog Number :

600-101-MR6S

Name :

Anti-hCG Alpha (GOAT) Antibody - 600-101-MR6S

Display Name :

hCG Alpha chain Antibody

Application Note :

Anti-Human Chorionic Gonadotropin (hCG) Alpha Antibody has been tested in ELISA and WB. Expect a band at ~14.9kDa for the alpha chain in western blot using appropriate tissues and lysates. ELISA reactivity expected against alpha hCG with an expected titer of ~1:100,000 and full hCG at ~1:50,000 dilution.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:20,000-1:100,000

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CGA

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Human Chorionic Gonadotropin Alpha Antibody was prepared from whole goat serum produced by repeated immunizations with Hu Chorionic Gonadotropin Alpha Subunit protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against hCG alpha. This product was affinity purified from monospecific antiserum by immunoaffinity purification. This product reacts with Full Human Chorionic Gonadotropin (hCG) and Human Chorionic Gonadotropin Alpha chain (α-hCG).

Species Reactivity :

Human

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

Goant Anti-Human Chorionic Gonadotropin Alpha Antibody, Goat anti-hCGa Antibody, α-hCG, a-hCG, alpha-hCG, Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, CG-alpha, Follicle-stimulating hormone alpha chain, FSH-alpha, Follitropin alpha chain, Luteinizing hormone alpha chain, LSH-alpha, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, TSH-alpha, Thyrotropin alpha chain

Target Species :

Human

Background :

Human Chorionic Gonadotropin (hCG) is a member of the glycoprotein hormone family which includes lutropin, follitropin, and thyrotropin. These hormones are heterodimers consisting of a common alpha subunit, and a unique beta chain which confers their biological specificity to the hormones. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways. HCG is secreted in large quantities by normal trophoblasts. Its presence is in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. CGA is extracellular and secreted, expressed in over 90 organs, but has its highest expression level in placenta. Anti-hCG alpha detects hCG alpha in human cells and tissue and could be useful in detecting cells and tumors of trophoblastic origin such as choriocarcinoma. Anti-hCG Antibody is useful for researchers interested in pregnancy, hormone research, and cancer research.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Yes

Application Text :

ELISA,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P01215

NCBI :

NP_000726.1

Primary Image Name :

Western Blot of Goat Anti-hCG Alpha Antibody

New Product :

Yes

more info or order :

Rockland Immunochemicals product webpage