S Opsin Antibody | Rockland Immunochemicals 600-101-MP7 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

S Opsin Antibody

catalog :

600-101-MP7

quantity :

100

price :

499.00 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

mouse

application :

immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunohistochemistry of Goat Anti-S Opsin Antibody. Tissue: Mouse Eye Tissue (retina). Antigen Retrieval: HIER using Citrate buffer for 20mins. Fixative: none. Primary Antibody: Anti-S Ospin at 1:100 for 30 mins at RT. Secondary Antibody: Donkey Anti-Goat HRP at 4µL/mL for 20mins at RT. Counterstain: Hematoxylin. Analysis Results: S opsin is expected to stain the outer rod cells of the retina, which it does at a dilution of 1:100.

product information

Catalog Number :

600-101-MP7

Name :

Anti-S Opsin (GOAT) Antibody - 600-101-MP7

Display Name :

S Opsin Antibody

Application Note :

Anti-S Ospin Antibody has been tested in ELISA and IHC. Expect a band at ~39kDa in western blot using appropriate tissues and lysates. Positive control used: Mouse eye tissue in Immunohistochemistry.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.1 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100

Default Unit :

µg

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:100

Expiration :

Expiration date is one (1) year from date of receipt.

Format :

IgG

Gene Name :

Opn1sw

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-S Opsin antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of mouse S Opsin conjugated to Keyhole Limpet Hemocyanin (KLH).

Other Performance Data :

The 1:100 H1-20 provides optimal staining with staining of structures in multiple layers. These layers have been shown to express S-opsin in the cited reference. For staining only in the outer segment layer, the 1:200 and 1:300 H2-20 appears to be ideal.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against mouse S Ospin. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse.

Species Reactivity :

Mouse

Tested Applications :

IHC

Suggested Applications :

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat Anti-Short-wave-sensitive opsin 1 Antibody, S opsin, Blue cone photoreceptor pigment, Blue-sensitive opsin, BOP, Short wavelength-sensitive cone opsin, Bcp, Opn1sw

Target Species :

Mouse

Background :

Short-wave-sensitive opsin 1 belongs to the G-protein coupled receptor 1 family, opsin subfamily. It encodes the blue cone pigment gene which is one of three types of cone photoreceptors responsible for normal color vision. Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. Required for the maintenance of cone outer segment organization in the ventral retina, but not essential for the maintenance of functioning cone photoreceptors. Involved in ensuring correct abundance and localization of retinal membrane proteins. May increase spectral sensitivity in dim light. Defects in this gene are the cause of tritan color blindness (tritanopia). Affected individuals lack blue and yellow sensory mechanisms while retaining those for red and green. Defective blue vision is characteristic. Anti-S Ospin Antibody is useful for researchers interested in Color Blindness, Metabolism of fat-soluble vitamins, and Peptide ligand-binding receptors.

Immunogen Type :

Conjugated Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2025 Price :

499.00 USD

UniProt :

P51491

NCBI :

NP_031564.1

Primary Image Name :

Immunohistochemistry of Goat Anti-S Opsin Antibody

more info or order :

Rockland Immunochemicals product webpage