Collagen Type IV Antibody | Rockland Immunochemicals 600-101-MN4 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Collagen Type IV Antibody

catalog :

600-101-MN4

quantity :

100

price :

459.00 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Goat Anti-Collagen Type IV Antibody. Cells: NIH/3T3 cells. Fixative: 4%PFA. Permeabilization: None. Primary Antibody: Anti-Collagen Type IV at 15µg/mL overnight at 2-8°C. Counterstain: Donkey Anti-Goat IgG Dylight™488 (p/n 605-741-125) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-Collagen Tye IV + Dylight™488. (C) Merge A+B. (D) secondary only. Expected localization: Extracellular, ER.

product information

Catalog Number :

600-101-MN4

Name :

Anti-Collagen Type IV (GOAT) Antibody - 600-101-MN4

Display Name :

Collagen Type IV Antibody

Application Note :

Anti-Collagen IV antibody has tested in ELISA, IF, and IHC. This antibody is suitable in western blot, expect a band at ~160kDa in western blot using appropriate tissues or lysates. Positive control used mouse placenta and mouse testes in immunohistochemistry, and NIH/3T3 in immunofluorescence.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.18 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100

Default Unit :

µg

ELISA Dilution :

Starting dilution 5 µg/ml

Immunohistochemistry Dilution :

1:100 - see expanded information below

IF Microscopy Dilution :

5-15 µg/mL

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

COL4A1

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Collagen IV antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 240-265 of mouse Collagen IV.

Other Performance Data :

Ideal Conditions for Collagen IV on Mouse and Placenta HIER pH 6.0 for 20 mins at 1:100 dilution using Horse anti Goat secondary and Proteinase k shows good staining on mouse placenta. Link here HIER pH 9.0 for 20 mins at 1:100 dilution using Horse anti Goat secondary and Proteinase k shows good staining on mouse testis . Link here

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against mouse Collagen IV. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and 78.6% identity with human.

Species Reactivity :

Mus musculus (Mouse)

Tested Applications :

ELISA; IF; IHC

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, alpha unit Collagen IV Type IV Collagen, Col4, Collagen alpha-1(IV) chain

Target Species :

Mouse

Background :

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagen Type IV proteins are integral components of basement membranes. This type of collagen is found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Anti-Collagen Type IV Antibody is ideal for researchers involved in extracellular matrix proteins and nephritis.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2023 Price :

459.00 USD

UniProt :

P02463

NCBI :

12826

Primary Image Name :

Immunofluorescence of Goat Anti-Collagen Type IV Antibody

more info or order :

Rockland Immunochemicals product webpage