Eaat2 Antibody | Rockland Immunochemicals 600-101-MN3 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Eaat2 Antibody

catalog :

600-101-MN3

quantity :

100 µg

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Goat Anti-Eaat2 Antibody. Cells: Rat Pups Brain heterogenous cells. Fixation: 4% PFA. Permeabilization: 0.3%Triton X-100. Primary Antibody: Anti-Eaat2 at 15µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 5µg/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) anti-Eaat2 and seconday, (C) merge A+B, (D) secondary only. Expected localization: plasma membrane >> extracellular > mitochondria, nucleus.

product information

Catalog Number :

600-101-MN3

Name :

Anti-Eaat2 (GOAT) Antibody - 600-101-MN3

Display Name :

Eaat2 Antibody

Application Note :

Anti-Eaat2 Antibody has been tested in ELISA, WB, and IF. Expect a band at ~62kDa in western blot using appropriate tissues and lysates. Positive control used: rat and mouse brain lysates in western blot and Rat Pups Brain heterogenous cells in immunofluorescence.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.05 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:50,000

IF Microscopy Dilution :

15µg/mL

Western Blot Dilution :

1:200

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Slc1a2

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Eaat2 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminal region of Rat Eaat2 surrounding aa1-25 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against rat Eaat2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, and rat.

Species Reactivity :

Rat, Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat Anti-Eaat2 Antibody, Goat Anti-Excitotoxic Amino Acid Transporter Antibody, Solute Carrier Family 1 Member 2, Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter) Member 2, Sodium-Dependent Glutamate/Aspartate Transporter 2, Toxcitatory Amino Acid Transporter, Glutamate/Aspartate Transporter II, EAAT2, Excitotoxic Amino Acid Transporter, EIEE41, GLT-1, HBGT, GLT1

Target Species :

Rat

Background :

SLC1A2 (Solute Carrier Family 1 Member 2), glutamate transporters, also known as excitatory amino acid transporters (EAATs), are sodium- and potassium- dependent members of the solute carrier family 6 (SLC1), widely distributed throughout the brain. These sodium-dependent, high-affinity amino acid transporter mediates the uptake of L-glutamate, L-aspartate, and D-aspartate functioning as a symporter that transports one amino acid molecule together with two or three Na (+) ions and one proton, in parallel with the counter-transport of one K (+) ion. It mediates the Cl (-) flux that is not coupled to amino acid transport avoiding the accumulation of negative charges due to aspartate and Na (+) symport. Eaat2 is essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate. Improper regulation of this gene is thought to be associated with several neurological disorders including Epileptic Encephalopathy, Early Infantile, 41 and Undetermined Early-Onset Epileptic Encephalopathy. Anti-Eaat2 Antibody is useful for researchers interested in transmembrane transporter activity, neuroscience research, and cell metabolism.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P31596

NCBI :

NP_001289018.1

Primary Image Name :

Immunofluorescence of Goat Anti-Eaat2 Antibody

more info or order :

Rockland Immunochemicals product webpage