BMAL1 Antibody | Rockland Immunochemicals 600-101-ML8 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

BMAL1 Antibody

catalog :

600-101-ML8

quantity :

100 µg

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Goat anti-BMAL1 Antibody. Cell Line: HeLa Cells. Fixative: 4%PFA. Peermeabilization: 0.3% Triton X-100. Primary Antibody: Anti-BMAL1 at 15µg/mL overnight at 2-8°C. Secondary antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 15µg/mL at RT for 1 hr. Nuclear Counterstain: DAPI. Image Staining: (A) DAPI. (B) Anit-BMAL1 + secondary. (C) merge A+B. (D) secondary only.

product information

Catalog Number :

600-101-ML8

Name :

Anti-BMAL1 (GOAT) Antibody - 600-101-ML8

Display Name :

BMAL1 Antibody

Application Note :

Anti-BMAL1 Antibody has been tested in ELISA, WB, and IF. Expect a band at ~67-69 kDa in western blot using appropriate lysates. Positive control used: HEK293T and Mouse Brain whole cell lysates in WB, and HeLa cells in IF.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.92 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:50,000

IF Microscopy Dilution :

15 µg/ml

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

ARNTL

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-BMAL1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human Bmal1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Other Performance Data :

User Optimized

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human Bmal1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse, rat, golden hamster, horse, gallus gallus, Pongo abelii, Tyto alba, and Nannospalax galili.

Species Reactivity :

human, mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat Anti-Aryl hydrocarbon receptor nuclear translocator-like protein 1 Antibody, Goat Anti-Bmal1 Antibody, Arnt3, Brain and muscle ARNT-like 1

Target Species :

Human

Background :

Arntl (Aryl Hydrocarbon Receptor Nuclear Translocator Like), also known as Bmal1, is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, Angelman Syndrome and altered circadian rhythm. Anti-Bmal1 Antibody is useful for researchers interested in Neuroscience research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

O00327 human

Primary Image Name :

Immunofluorescence of Goat anti-BMAL1 Antibody

more info or order :

Rockland Immunochemicals product webpage