EIF2A Antibody | Rockland Immunochemicals 600-101-ML7 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

EIF2A Antibody

catalog :

600-101-ML7

quantity :

100 µg

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Goat Anti-EIF2A Antibody. Cells: HeLa Cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-EIF2A at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-EIF2A and DyLight™488. (C) merge of A+B. (D). Secondary only. Localization: cytosol, extracellular >> mitochondria.

product information

Catalog Number :

600-101-ML7

Name :

Anti-EIF2A (GOAT) Antibody - 600-101-ML7

Display Name :

EIF2A Antibody

Application Note :

Anti-EIF2A Antibody has been tested in ELISA, WB, and IF. Expect a band at ~65kDa in western blot using appropriate tissues and lysates. Positive control used: HeLa in WB and IF.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.08 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:50,000

IF Microscopy Dilution :

5µg/mL

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

EIF2A

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-EIF2A antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human EIF2A conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human EIF2A. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 86.7% homology with the immunizing sequence to mouse.

Species Reactivity :

Human, Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat Anti-Eukaryotic Translation Initiation Factor 2A Antibody, Eukaryotic Translation Initiation Factor 2A 65kDa, 65 KDa Eukaryotic Translation Initiation Factor 2A, EIF-2A, MSTP004, MSTP089, MST089, CDA02

Target Species :

Human

Background :

EIF2A (Eukaryotic Translation Initiation Factor 2A) functions in the early steps of protein synthesis of a small number of specific mRNAs. It acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. Not be confused with eIF2-alpha (EIF2S1), the alpha subunit of the eIF2 translation initiation complex. The eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner, whereas in contrast, EIF2A binds methionyl-tRNAi to 40S subunits in a codon-dependent manner. Goat Anti-EIF2A Antibody is useful for researchers interested in phototoxic stress, translation initiation factor activity, and ribosome binding.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9BY44

NCBI :

NP_001305972.1

Primary Image Name :

Immunofluorescence of Goat Anti-EIF2A Antibody

more info or order :

Rockland Immunochemicals product webpage