Anti-PI3 Kinase p55 gamma Antibody | Rockland Immunochemicals 600-101-ML2 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Anti-PI3 Kinase p55 gamma Antibody

catalog :

600-101-ML2

quantity :

100 µg

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Goat Anti-PIK3R3 Antibody. Cell Line: A431 Cells. Fixation: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-PI 3 Kinase p55 gamma Antibody at 15µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 15µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Localization: Cytosol (UniProtKB), Nucleoplasms (HPA) distinct punctate staining in nucleus could indicate nucleoplasm. Staining: (A) DAPI, (B) Anti-PIK3R3 + secondary, (C) A+B merge, (D) secondary only.

product information

Catalog Number :

600-101-ML2

Name :

Anti-PI3 Kinase p55 gamma (PIK3R3) (GOAT) Antibody - 600-101-ML2

Display Name :

Anti-PI3 Kinase p55 gamma Antibody

Application Note :

Anti-PI3 Kinase p55 gamma Antibody has been tested in ELISA, WB, IF, and IHC. Expect bands ~44, 47, 54kDa in western blot using appropriate tissues or lysates. Positive control used: mouse testis in WB; A431 cells in IF; Human testis and Human tonsil tissue in IHC.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.02 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000 - 50,000

Immunohistochemistry Dilution :

1:100

IF Microscopy Dilution :

15 µg/ml

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

PIK3R3

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-PI3 Kinase p55 gamma antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-Terminal portion of human PI3 Kinase p55 gamma conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human PI 3 Kinase p55 gamma. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and rat, 94.1% homology to bovine.

Species Reactivity :

human, mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat Anti-PI3 Kinase p55 gamma Antibody, Goat Anti-PIK3R3 Antibody, Anti-p55 gamma Antibody, Phosphoinositide-3-Kinase Regulatory Subunit 3, Phosphatidylinositol 3-Kinase 55 KDa Regulatory Subunit Gamma, Phosphoinositide-3-Kinase, Regulatory Subunit 3 (Gamma), PtdIns-3-Kinase Regulatory Subunit P55-Gamma, PI3-Kinase Regulatory Subunit Gamma, PI3K Regulatory Subunit Gamma, PI3-Kinase Subunit P55-Gamma, P55PIK, Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 3 (P55, Gamma), Phosphatidylinositol 3-Kinase Regulatory Subunit Gamma, Phosphoinositide-3-Kinase Regulatory Subunit Gamma, PtdIns-3-Kinase Regulatory Subunit Gamma, P55-GAMMA, P55

Target Species :

Human

Background :

PI 3-Kinases (phosphoinositide 3-kinases, PI 3-Ks) are a family of lipid kinases capable of phosphorylating the 3'OH of the inositol ring of phosphoinositides. They are responsible for coordinating a diverse range of cell functions including proliferation and survival. PIK3R3 (Phosphoinositide-3-Kinase Regulatory Subunit 3) binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Anti-PIK3R3 Antibody is useful for researchers interested in cancer research, Asbestos-related lung carcinomas, intravenous Leiomyomatosis, ERK signaling, and mTOR Pathways.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q92569

NCBI :

NP_001107644.1

Primary Image Name :

Immunofluorescence of Goat Anti-PIK3R3 Antibody

more info or order :

Rockland Immunochemicals product webpage