Brn3b Antibody | Rockland Immunochemicals 600-101-MJ0S product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Brn3b Antibody

catalog :

600-101-MJ0S

quantity :

25 µL

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence of Goat Anti-Brn3b Antibody. Cell Line: Post-natal rat pup (PND1) heterogeneous brain cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Brn3b at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 15µg/mL for 1 hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Brn3b + secondary. (C). Merge A+B. (D). secondary only. Expected localization: Nuclear, nuclear speckle.

product information

Catalog Number :

600-101-MJ0S

Name :

Anti-Brn3b (GOAT) Antibody - 600-101-MJ0S

Display Name :

Brn3b Antibody

Application Note :

Anti-Brn3b Antibody has been tested in ELISA, WB, IHC, and IF. Expect a band at ~43kDa in western blot using appropriate lysates. Positive controls used: Rat pup PND2-6 – minimal cortex brain lysate in WB, Mouse testis in IHC, Post-natal rat pup (PND1) heterogeneous brain cells in IF.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.05 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:10,000 - 50,000

Immunohistochemistry Dilution :

1:100

IF Microscopy Dilution :

5 µg/ml

Western Blot Dilution :

1:1000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

Pou4f2

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Brn3b antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Pou4f2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against mouse Brn3b. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat and 91.7% to human.

Species Reactivity :

Mouse, rat

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

Goat Anti-Brn3b Antibody, Goat Anti-Pou4f2 Antibody, POU domain, class 4, transcription factor 2, Brain-specific homeobox/POU domain protein 3B, Brn-3B, Brain-3B, Brn-3.2

Target Species :

Mouse

Background :

Brn3b (Brain-Specific Homeobox/POU Domain Protein 3B) or POU4F2 (POU Class 4 Homeobox 2) is a DNA-binding transcriptional regulator and coregulator that recognizes and binds to the consensus octamer binding site 5-AT[A/T]A[T/A]T[A/T]A-3 in promoter of target genes. Brn3b plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor ISL1 to achieve RGC fate specification in the developing retina. And plays a role in RGC axon formation and guidance by regulating gene expression of specific target genes. Brn3b plays a role in TNFSF11-mediated terminal osteoclast differentiation. It binds chromatin at promoter region of target genes. It acts either as a transcriptional activator or repressor. It plays a role in the regulation of breast cancer cell growth by promoting transcription activation as well as repression of specific target genes. It is involved in tumor breast progression and invasion. Brn3b also plays a role either as a transcriptional coactivator or corepressor. Anti-Brn3b Antibody is useful for researchers interested in breast cancer research, transcription factor activity, and DNA binding.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q63934

NCBI :

NP-620394.2

Primary Image Name :

Immunofluorescence of Goat Anti-Brn3b Antibody

more info or order :

Rockland Immunochemicals product webpage