VDAC/Porin Antibody | Rockland Immunochemicals 600-101-HB2 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

VDAC/Porin Antibody

catalog :

600-101-HB2

quantity :

100

price :

425.00 USD

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Reference
Vo M, Choi C, Choi Y. The mitophagy receptor NIX induces vIRF-1 oligomerization and interaction with GABARAPL1 for the promotion of HHV-8 reactivation-induced mitophagy. PLoS Pathog. 2023;19:e1011548 pubmed publisher

image

image 1 :

Immunofluorescence of Goat Anti-VDAC/Porin Antibody. Cell Line: HeLa Cells. Fixation: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-VDAC1/Porin at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 15µg/mL for 1hr at RT. Nuclear counter stain: DAPI. Location: Mitochondrion, Mitochondrion Outer Membrane, Nucleus. A). DAPI. B). VDAC/DyLight™488. C). Merge of A+B. D). Secondary Only.

product information

Catalog Number :

600-101-HB2

Name :

Anti-VDAC/Porin (GOAT) Antibody - 600-101-HB2

Display Name :

VDAC/Porin Antibody

Application Note :

VDAC/Porin Antibody has been tested for use in ELISA, western blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract. Positive control used in WB: HeLa Whole Cell, Mouse Heart Lysate, or Rat Brain Tissue Lysate, and HeLa Cells in IF.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.92 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100

Default Unit :

µg

ELISA Dilution :

1:10,000-1:50,000

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

5 µg/ml

Western Blot Dilution :

1 µg/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

VDAC1

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

VDAC/Porin Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal portion of Human VDAC1/Porin1.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 including mouse, rat, bovine, pig, and rabbit sources based on 100% homology for the immunogen sequence. Such broad reactivity makes this antibody useful as an excellent loading control.

Species Reactivity :

H. sapiens (Human); Mus musculus (Mouse); Rattus (Rat)

Tested Applications :

ELISA; IF; WB

Storage :

Store VDAC antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat anti-VDAC/Porin antibody, VDAC-1 Loading Control Antibody, Porin Loading Control Antibody, VDAC-1 Antibody, VDAC1 Antibody, POR1 Antibody, Porin Antibody, Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL

Target Species :

Human

Background :

VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. Diseases associated with VDAC1 include Cystic Fibrosis and Gerstmann-Straussler Disease. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations. Anti-VDAC/Porin Antibody is useful for researchers interested in Apoptosis, cell survival and regulation, Mitochondrial Proteins, and Neuroscience Research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2023 Price :

425.00 USD

UniProt :

P21796

NCBI :

7416

Primary Image Name :

Immunofluorescence of Goat Anti-VDAC/Porin Antibody

more info or order :

Rockland Immunochemicals product webpage