PDL1 Antibody | Rockland Immunochemicals 600-101-GU8 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PDL1 Antibody

catalog :

600-101-GU8

quantity :

100 µg

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

Immunofluorescence Microscopy of Goat anti-PDL1 antibody. Cell line: MDA-MB-231 p/n (W09-001-GK6). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PLD1 antibody at 10 µg/mL for overnight at 4°C. Secondary antibody: Anti-Goat IgG DyLight™488 (Rabbit) Conjugated Preadsorbed (p/n 605-441-013) at 5 ug/ml for 2 hrs at RT. Localization: PDL1 is located in the cell membrane and cytoplasm. Staining: PDL1 as green fluorescent signal with DAPI (blue) nuclear counterstain.

product information

Catalog Number :

600-101-GU8

Name :

Anti-PDL1 (GOAT) Antibody - 600-101-GU8

Display Name :

PDL1 Antibody

Application Note :

Anti-PDL-1 Antibody has been tested in Western Blot, ELISA, and IF. Positive control whole cell lysates used MDA-MB-231 p/n (W09-001-GK6) and A549 p/n (W09-001-372) and positive recombinant protein control for western blotting. Expect a band at ~33.3 kDa in western blot using appropriate lysates. Immunofluorescence positive control cell line used was MDA-MB-231 p/n (W09-001-GK6). Although not tested, this antibody is likely functional in flow cytometry and immunoprecipitation.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.07 mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µg

Default Unit :

µg

ELISA Dilution :

1:10,000-1:50,000

Flow Cytometry Dilution :

User Optimized

Immunohistochemistry Dilution :

1:200

IF Microscopy Dilution :

10µg/mL

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1.0 ug/ml

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CD274

Host Animal :

Goat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-PDL1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human PDL1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity purified antibody is directed against human PDL1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.

Species Reactivity :

Human, Mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Goat anti-PDL1 antibody, Programmed cell death 1 ligand 1, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, PD-L1, PDCD1 ligand 1, B7-H1, B7 homolog 1, PDL-1, CD274 molecule antibody, MGC142294 antibody, PDL 1

Target Species :

Human

Background :

PDL1 belongs to the immunoglobulin superfamily and BTN/MOG family. PDL1 is involved in the costimulatory signal, essential for T-cell proliferation and production of IL-10 and IFNG, in an IL-2 dependent and a PDCD1 independent manner. It is up-regulated in T-and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. PDL1 is up-regulated in B-cells activated by surface Ig cross-linking. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Anti-PDL-1 Antibody is useful for researches interested in cancer research, NF-kB pathway research, and the Immune system.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9NZQ7

NCBI :

NP_001254635.1

Primary Image Name :

Immunofluorescence Microscopy of Goat anti-PDL1 antibody

more info or order :

Rockland Immunochemicals product webpage